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| Mechanism of RUNX1 Regulating Migration and Invasion of Laryngeal Squamous Cell Carcinoma Cells |
| DING Wenyu, CHANG Xiao, YANG Yifei |
| The Affiliated Hospital of Hebei Engineering University, Hebei Handan 056000, China |
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Abstract Objective: To explore the regulatory mechanism of runt related transcription factor 1 (Runx1) on the proliferation, cell cycle, apoptosis, migration and invasion of laryngeal squamous cell carcinoma (LSCC) Hep2 cells. Methods: Si-con group (transfected Si con) and Si-RUNX1 group (transfected Si-RUNX1) were transfected into Hep2 cells by liposome method; Real time fluorescence quantitative reverse transcription polymerase chain reaction (RT qPCR), western blotting (WB), cell counting kit 8 (CCK8), 5-ethynyl-2 '- deoxyuridine (EDU), flow cytometry, scratch test Transwell assay was used to detect the mRNA and protein expression of Runx1, cell proliferation, cell cycle, apoptosis, cyclin D1 (cyclin D1), cyclin D-dependent kinase (CDK4), cleaved caspase-3, bcl-2 related X protein (Bax), B lymphoma-2 (Bcl-2), protein expression of matrix metalloproteinase (MMP) - 2, MMP-9, phosphatidylinositol-3-kinase (PI3K), protein kinase B (Akt) and phosphorylation (P) - Akt. Results: Compared with Si-con group, the expression of Runx1 mRNA and protein, cell proliferation, S-phase arrest of cell cycle, apoptosis rate, scratch healing rate and the number of migrating and invading cells in Si-RUNS1 group were significantly decreased (P<0.05). Importantly, the intervention of RUNX1 also inhibited the protein expression of PI3K, p-Akt and p-Akt / Akt, the key factors of PI3K / Akt signaling pathway. Conclusion: The intervention of Runx1 is not conducive to the survival, migration and invasion of Hep2 cells, and its mechanism may be related to the inhibition of the activity of PI3K / Akt signaling pathway.
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2022, Vol. 28 
