大黄素通过上调miR-1301抑制胰腺癌SW1990细胞的增殖迁移和侵袭

doi:10.3969/j.issn.1006-6233.2022.05.05

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Abstract Objective: To explore the roles of emodin on proliferation, migration and invasion of pancreatic cancer SW1990 cells and its molecular mechanism. Methods: Human pancreatic ductal epithelial cells HPDE6 and pancreatic cancer SW1990 cells were cultured in vitro, and SW1990 cells were divided into control group, low-dose emodin group, medium-dose emodin group, high-dose emodin group, miR-NC group, miR-1301 group, high-dose emodin+anti-miR-NC group, high-dose emodin+anti-miR-1301 group. Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the expressions of miR-1301. The tetramethylazozolate colorimetric method (MTT) was implemented to measure cell activity. The protein expression was assessed by Western blot. Transwell was employed to monitor cell migration and invasion. Results: Compared with human pancreatic ductal epithelial cells HPDE6, the expression level of miR-1301 in pancreatic cancer SW1990 cells was significantly decreased(P<0.05). Compared with the control group, medium and high-doses of emodin significantly decreased the activity, migration and invasion ability and the levels of CyclinD1, MMP-2 and MMP-9, and increased the level of p21 of pancreatic cancer SW1990 cells(P<0.05). Compared with the control group, the expression levels of miR-1301 in medium and high-dose emodin groups were significantly increased(P<0.05). Overexpression of miR-1301 significantly reduced the activity, migration and invasion ability and the levels of CyclinD1, MMP-2 and MMP-9, and increased the level of p21 of pancreatic cancer SW1990 cells(P<0.05). Down-regulation of miR-1301 reversed the roles of emodin on proliferation, migration and invasion of pancreatic cancer SW1990 cells(P<0.05). Conclusion: Emodin may inhibit cell proliferation, migration and invasion by up-regulating the expression of miR-1301 in SW1990 cells.

Key words： Emodin miR-1301 Proliferation Migration Invasion

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.05.05 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I5/725

[1] Freddie B,Jacques F,IsabellE S,et al.Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer Clin,2018,68(6):394~424.
[2] Yu Y W,Tong Y,Zhong A L,et al.Identification of serum microRNA-25 as a novel biomarker for pancreatic cancer[J].Medicine (Baltimore),2020,99(52):e23863.
[3] Li-weber M.Targeting apoptosis pathways in cancer by Chinese medicine[J].Cancer Lett,2013,332(2):304~312.
[4] LIN S Z,XU J B,JI X,et al.Emodin inhibits angiogenesis in pancreatic cancer by regulating the transforming growth factor-β/drosophila mothers against decapentaplegic pathway and angiogenesis-associated microRNAs[J].Mol Med Rep,2015,12(4):5865~5871.
[5] Liu Q,Hodge J,Wang J F,et al.Emodin reduces breast cancer lung metastasis by suppressing macrophage-induced breast cancer cell epithelial-mesenchymal transition and cancer stem cell formation[J].Theranostics,2020,10(18):8365~8381.
[6] Jin Y Z,Sun H N,Liu Y,et al.Peroxiredoxin V inhibits emodin-induced gastric cancer cell apoptosis via the ROS/Bcl2 pathway[J].In Vivo,2019,33(4):1183~1192.
[7] Liu C,Chen L,Wang W,et al.Emodin suppresses the migration and invasion of melanoma cells[J].Biol Pharm Bull,2021,44(6):771~779.
[8] TrybusS W,Krol T,Trybus E,et al.Emodin induces death in human cervical cancer cells through mitotic catastrophe[J].Anticancer Res,2019,39(2):679~686.
[9] Yang F,Wang H,Yan B,et al.Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway[J].Biol Chem,2021,402(7):805~813.
[10] Chen X,Sun H,Zhao Y,et al.CircRNA circ_0004370 promotes cell proliferation,migration,and invasion and inhibits cell apoptosis of esophageal cancer via miR-1301-3p/COL1A1 axis[J].Open Med (Wars),2021,16(1):104~116.
[11] Bai J G,Wu J F,Tang R F,et al.Emodin,a natural anthraquinone,suppresses liver cancer in vitro and in vivo by regulating VEGFR and miR-34a[J].Invest New Drugs,2020,38(2):229~245.
[12] Song K J,Lv T,Chen Y L,et al.Emodin inhibits TGF-β2 by activating the FOXD3/miR-199a axis in ovarian cancer cells in vitro[J].Oncol Rep,2018,39(5):2063~2070.