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| SNHG15-lncRNA CeRNA Network Targets MiR-451a to Promote the Proliferation of Glioma |
| WU Weichuan, MEN Donghai, LIU Leifeng, et al |
| The Affiliated Hospital of Guangdong Medical University, Guangdong Zhanjiang 524000, China |
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Abstract Objective: To explore the mechanism of SNHG15-lncRNA ceRNA network to promote glioma proliferation by targeting miR-451a. Methods: The expression levels of SNHG15 and miR-451a in clinical tissue samples, human glial cell lines HEB, and glioma cell lines (U87, U251 and Hs683) were detected by qRT-PCR. Dual luciferase reporter gene assay, qRT-PCR and Pull down were used to detect the targeted regulation of SNHG15-miR-451a axis. U87 cells were transfected with SNHG15 small interfering RNA (siRNA), negative control sequence (NC) and miR-451a inhibitor. The cells were randomly divided into siNC group, siSNHG15 group and siSNHG15+miR-451a inhibitor group. The regulation of invasion, migration and apoptosis of U87 cells by SNHG15 and miR-451a were detected by Transwellcell invasion, cell scratching and Tunel cell apoptosis, respectively. A subcutaneous transplantation tumor model of glioma in nude mice was constructed to detect tumor growth and cell proliferation in vivo. Results: Compared with the adjacent tissues and HEB cells, the expression of SNHG15 in glioma tissues and glioma cell lines was significantly increased, while the expression of miR-451a was significantly decreased (all P<0.05). Dual luciferase reporter gene results showed that SNHG15 luciferase activity increased significantly after transfection with miR-451a inhibitor (P<0.05). Pull down biotinylation assay showed that miR-451a decreased the expression level of SNHG15 in U87 cells (p<0.05), while the expression of miR-451a was decreased after transfection with SNHG15 plasmid (P<0.05). SNHG15 significantly inhibited, while miR-451a inhibitor significantly promoted the invasion, migration distance and apoptosis rate of U87 cells (all P<0.05). The SNHG15 group had a significantly higher graft weight than the NC group, while the miR-451a group had a significantly lower graft weight than the miR-NC group (both P<0.05) The expression of PCNA in siSNHG15 group was lower than in siNC group and siSNHG15+miR-451a inhibitor group (both P<0.05). The expression of PCNA in miR-451a inhibitor group was higher than in NC inhibitor group and siSNHG15+miR-451a inhibitor group (all P<0.05). Conclusions: SNHG15 competitively binds miR-451a to promotes the invasion and migration of glioma cell, and inhibits apoptosis. Down-regulating the SNHG15-miR-451a axis might serve as a new therapeutic target for glioma treatment.
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2022, Vol. 28 
