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| Effect of Transfection of ELF5 mRNA on Apoptosis and Cycle of Endometrial Carcinoma Cells |
| QI Ting, et al |
| Graduate School of Xuzhou Medical University, Jiangsu Xuzhou 221000, China |
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Abstract Objective: To study the effects of ELF5 gene on cycles and apoptosis of human endometria cancer cells and its probable mechanism. Methods: The eukaryotic expression vectedical or pcDNA3.1-ELF5+EGFP was transfected into human endometria cancer cells in vitro (recombinant plasmid group), and positive cell clones were selected by G418 and amplified. Transfected pcDNA3.1-EGFP cells (empty plasmid group) and untransfected cells (blank control group) were served as control groups. In vitro, the cell prolifetraion ability of three groups was analyzed by MTT; the cycle and apoptosis was analyzed by flow cytometry; the expression of protein P21 and Caspase-3 was detected by Western blot. In vivo, three groups of cells were transplanted into NOD/SCID nude mice, and observed the growth ability of tumors; the apoptosis of tumors was determined by TUNEL; the expression of protein P21 and Caspase-3 in tumors was detected by Western blot. Results: The recombinant plasmid pcDNA3.1- ELF5+EGFP was successfully transfected into human endometria cancer cells; The expression of ELF5 mRNA in the recombinant plasmid group was higher than that in the empty plasmid group and blank control group (P<0.05); At each time point, the cell proliferation ability of the recombinant plasmid group was significantly decreased than that of the empty plasmid group and blank control group, the differences were statistically significant (P<0.05); The number of cells arrested in G0/G1 phase of the recombinant plasmid group was higher than that of the empty plasmid group and blank control group, the differences were statistically significant (P<0.05); The apoptosis rate of the recombinant plasmid group was compared with the empty plasmid group and the blank control group, the differences were statistically significant (P<0.05); The expression of protein P21 and Caspase-3 in the recombinant plasmid group was higher than that in the empty plasmid group and blank control group (P<0.05). In vivo, the growth ability of tumors (maximum diameter, weight) in recombinant plasmid group was lower than that in the empty plasmid group and blank control group, the differences were statistically significant (P<0.05); In vivo, the apoptosis rate of the recombinant plasmid group was compared with the empty plasmid group and the blank control group, the differences were statistically significant (P<0.05); In vivo, the expression of protein P21 and Caspase-3 in the recombinant plasmid group was higher than that in the empty plasmid group and blank control group (P<0.05). Conclusions: ELF5 gene has obvious effects on cycles and apoptosis of human endometria cancer cells and its mechanism connected with the protein P21 and Caspase-3.
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2017, Vol. 23 
