促红细胞生成素对大鼠磨牙牙髓血运重建术后SDF-1和VEGF表达水平及疼痛的影响

doi:10.3969/j.issn.1006-6233.2022.02.09

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Abstract Objective: To investigate the effects of EPO (erythropoietin) on the expression of SDF-1 and VEGF and pain after pulp revascularization in molar rats. Methods: Totally 48 rats were randomly divided into normal group, model group (molar pulp revascularization rats), EPO-A group (molar pulp revascularization rats intraperitoneal injection of 250IU/Kg EPO) and EPO-B group (molar pulp revascularization rats intraperitoneal injection of 500IU/Kg EPO). ELISA method was used to detect the expression of TNF-α, IL-6 and IL-1β in the serum of each group of rats. HE staining was used to observe the morphology of root canal tissue. Western blotting was used to detect the protein levels of SDF-1 and VEGF in the root canal tissue. Human dental pulp cells were divided into normal group (human dental pulp cells), EPO group (human dental pulp cells given EPO intervention), VEGF group (human dental pulp cells given VEGF intervention) and SDF-1 group (human dental pulp cells given SDF-1 intervention). CCK-8 was used to detect the relative proliferation rate of 24h, 48h and 72h cells. qRT-PCR detects the relative expression of SDF-1 and VEGF mRNA. Results: Compared with the normal group, the serum levels of TNF-α, IL-6 and IL-1β in the model group increased (P<0.05).Compared with the model group, the inflammatory indexes in the serum of rats in the EPO-A group and EPO-B group were reduced (P<0.05), and the EPO-B group was lower than the EPO-A group (P<0.05); the expression level of SDF-1 and VEGF protein in the root canal tissue of the model group was lower than that of the normal group (P<0.05).The protein expression levels of SDF-1 and VEGF in the EPO-A and EPO-B groups were higher than those in the model group (P<0.05), and there was a significant difference between the EPO-B group and the EPO-A group (P<0.05). When the human dental pulp cells were cloned to the second generation, the morphology of the cells was observed under inverted microscope. It was found that the cells were in good growth condition, showing spindle shape or star shape. The cells were closely arranged, the cytoplasm was abundant, the processes of the cytoplasm were interconnected, and the nuclei were oval and located in the center of the cells, with obvious nucleoli. The relative proliferation rate of human dental pulp cells in EPO group, SDF-1 group and VEGF group at 24h, 48h and 72h was higher than that in normal group (P<0.05). Compared with the normal group, the mRNA expressions of SDF-1 and VEGF in EPO, SDF-1 and VEGF groups were increased (P<0.05), but the mRNA levels of SDF-1 and VEGF in EPO, SDF-1 and VEGF groups were not significant (P>0.05). Sdf-1 mRNA was positively correlated with VEGF mRNA (r=0.576, P=0.003). Conclusion: Eerythropoietin can reduce IL-1Β level and relieve pain after molar pulp revascularization in rats, and promote angiogenesis to participate in revascularization by up-regulating the expression of SDF-1 and VEGF.

Key words： Erythropoietin Revascularization Vascular endothelial growth factor Stromal cell derived factor

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.02.09 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I2/224

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