|
|
|
| Comparative Study of Dental Pulp Stem Cells Intravenous Injection and Combined Core Decompression in the Treatment of Early Steroid-Associated Femoral Head Necrosis in Rabbits |
| LU Yang, LIU Fei, WANG Xinmin, et al |
| The First Hospital of Qinhuangdao, Hebei Qinhuangdao 066000, China |
|
|
|
|
Abstract Objective: To study the therapeutic effect of intravenous application of dental pulp stem cells on early steroid-associated femoral head necrosis in rabbits, and to provide experimental data support for clinical application. Methods: Forty seven healthy adult Japanese white rabbits were randomly divided into 4 groups. There were 5 rats in group A (normal control group), 14 rats in group B (model group), 14 rats in group C (intravenous administration group) and 14 rats in group D (stem cell transplantation group). Models of femoral head necrosis were built among group A, B, and C before relative treatment was conducted to every of the three groups. After 12 weeks of modeling, the X-ray, micro CT, tissue sections and immunohistochemical indexes were collected and statistically analyzed. Results: There were significant differences between group C and group D in bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (TB.N), structural model index (SMI), bone mineral density (BMD), void lacuna rate, trabecular area ratio and VEGF (P<0.05). The indicators of stem cell transplantation group(group D) were better than those of intravenous administration group(group C). Conclusion: Both intravenous injection of dental pulp stem cells and intratunnel injection of dental pulp stem cells after core decompression are effective in the treatment of steroid induced femoral head necrosis in rabbits, and the effect of stem cell transplantation is better than that of intravenous injection.
|
|
|
|
|
|
[1] Cui L,Zhuang Q,Lin J,et al. Multicentric epidemiologic study on six thousand three hundred and ninety five cases of femoral head osteonecrosis in China[J]. Int Orthop,2016,40(2):267~276.
[2] 曾祥洪,梁博伟.股骨头坏死保髋治疗的新策略[J]. 中国组织工程研究,2021,25(03):431~437.
[3] Miyanishi K, Yamamoto T, Irisa T, et al. Effects of different corticosteroids on the development of osteonecrosis in rabbits[J].Rheumatology(oxford),2004,4(3):332~336.
[4] 张长青. 股骨头坏死保髋治疗指南(2016 版)[J]. 中华老年骨科与康复电子杂志,2016,2(2):65~70.
[5] Zhang QY, Li ZR, Gao FQ, et al.Pericollapse stage of osteonecrosis of the femoral head:a last chance for joint preservation[J].Chin Med,2018,131(21):2589~2598.
[6] Sultan AA,Khlopas A,Surace P,et al.The use of non-vascularized bone grafts to treat osteonecrosis of the femoral head indications, techniques, and outcomes[J].Int Orthop,2019,43 (6):1315~1320.
[7] Gangji V,De Maertelaer V,Hauzeur JP.Autologous bone marrow cell implantation in the treatment of non-traumatic osteonecrosis of the femoral head: five year follow-up of a prospective controlled study[J]. Bone,2011,49(5):1005~1009.
[8] Ayoub S,Berberi A,Fayyad-Kazan M.An update on human periapical cyst-mesenchymal stem cells and their potential applications in regenerative medicine[J].Mol Biol Rep,2020,47(3):2381~2389.
[9] Heng B C,Zhu S,Xu J,et al.Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitro[J].Tissue Cell,2016,48(2):133~143. |
|
|
|
2022, Vol. 28 
