血清miRNA-21 IL-6联合检测对心肺复苏成功患者预后的评价

doi:10.3969/j.issn.1006-6233.2021.12.031

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Abstract Objective: To study the importance of united gauging of serum microRNA-21 (miRNA-21) , interleukin -6 (IL-6) and echocardiography on cardiac function and prognosis of patients after cardiopulmonary resuscitation (CPR). Methods: 80 patients with cardiopulmonary arrest receiving successful CPR and 72 healthy participants were recruited in the study. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and ELISA was used to examine the serum miRNA-21 level and IL-6.Echocardiography is used to assess cardiac function after cardiopulmonary resuscitation. Spearman correlation was used to analyze the correlation between serum miRNA-21 and IL-6 expression levels and cardiac function. Patients in the case group were followed up for half a year. The end point of the follow-up was heart failure readmission or all-cause death. ROC curve was plotted to analyze the value of miRNA-21 and IL-6 expression levels in the prognosis assessment of patients after cardiopulmonary resuscitation. Results: Compared with the control group, serum miRNA-21 expression level and IL-6、pro-BNP level in the case group were significantly increased, left ventricular end diastolic diameter (LVEDD) increased, and left ventricular ejection fraction (LVEF) descended (P<0.05). In addition, Spearman correlation analysis displayed that the standard of miRNA-21 and IL-6 was negatively connected with cardiac function index(r =- 0.722, 0.613, P = 0.007, 0.009). Stepwise regression analysis showed that miRNA-21 and IL-6 were independent risk factors for readmitted and death after cardiopulmonary resuscitation. The area under the ROC curve of miRNAa-21 and IL-6 combined for prognosis assessment of patients after cardiopulmonary resuscitation was 0.937 and the 95% confidence interval was 0.853 ~ 1.000. Conclusion: The elevation of miRNA-21 and IL-6 level after CPR is closely related to the severity of cardiac dysfunction detected by ultrasound and the prognosis of patients, suggesting that miRNA-21、IL-6 may serve as a potential biomarker.

Key words： Cardiopulmonary resuscitation MiRNA-21 IL-6 Echocardiography Prognosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.12.031 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I12/2075

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