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| COX-2/PGE2 Activates the JAK/STAT3 Signaling Pathway to Regulate the Proliferation Invasion and Metastasis of KB Cells in Human Oral Cancer |
| ZHOU Tao, QIAN Yong, LIU Weisong, et al |
| Hainan Cancer Hospital, Hainan Haikou 570312, China |
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Abstract Objective: To investigate the expression of COX-2 in human oral cancer KB cells and its mechanism of regulating the proliferation, invasion and metastasis of cancer cells. Methods:The differential expression of COX-2 in human oral cancer and adjacent normal tissues, human oral cancer KB cells and human oral epithelial HIOEC cells were detected by qRT-PCR and Western blot. The KB cell line with high COX-2 expression was established by lentivirus transfection or COX2 inhibitor (celecoxib) was added, the expression levels of COX-2 in KB cell lines were detected by RT-qPCR and Western blot, CCK-8 assay was used to detect cell proliferation, Transwell assay was used to detect cell invasion, and Western blot was used to analyze the expression of EMT-related proteins in cells,PGE2 content in cell culture medium was detected by ELISA, and the expression levels of JAK, P-JAK, STAT3 and P-STAT3 proteins were detected by Western blot. Proliferation, invasion, and EMT of KB cells were measured after PGE2 receptor PTGER2 inhibitor (AH6809)or JAK/STAT3 pathway inhibitor (AG490) acted on KB cell lines. Results:The expression of COX-2 in human oral cancer tissues was significantly higher than that in adjacent normal tissues(P<0.05). Compared with HIOEC cells, the expression of COX-2 in KB cells was significantly increased (P<0.01). KB cell lines with high COX-2 expression were successfully constructed. Overexpression of COX-2 promoted proliferation, invasion and EMT of KB cells, significantly increased the expression of p-JAK and p-STAT3 proteins, and up-regulated the expression of PGE2 in cell culture medium. AH6809 or AG490 could significantly inhibit the proliferation, invasion and EMT of KB cells. Conclusion:COX-2/PGE2 activates the JAK/STAT3 signaling pathway to promote the proliferation, invasion and EMT of KB cells in human oral cancer.
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2021, Vol. 27 
