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| Effect of Dahuang Mudan Decoction on Lipopolysaccharide-induced Pyrosis of Intestinal Epithelial Cells and NLRP3/caspase-1 Pathway |
| HE Haifeng, et al |
| Shaanxi Provincial Hospital of Traditional Chinese Medicine, Shaanxi Xi'an 720082, China |
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Abstract Objective: To investigate the effect of Dahuang Mudan Decoction on lipopolysaccharide (LPS)-induced pyrosis of intestinal epithelial cells and the influence on nucleotide binding oligomerization domain-like receptor family pyrin domain containing 3/caspase-1 (NLRP3/caspase-1) pathway. Methods: Intestinal epithelial cells were randomly divided into control group, LPS group and rhubarb Mudan Decoction low, medium and high dose groups (2.52, 5.04 and 7.56 g / kg). Blank serum was added to the control group, LPS and blank serum were added to the LPS group, and LPS and medicated serum were added to the rhubarb Mudan Decoction group. The activity of intestinal epithelial cells was detected by thiazole blue (MTT), the apoptosis of intestinal epithelial cells was detected by flow cytometry, the expression levels of NLRP3, caspase-1 and gsdmd-c were detected by Western blot (WB), the expression of NLRP3 in cells was detected by immunofluorescence, and the pro-inflammatory factor interleukin (IL) - 1 was detected by enzyme-linked immunosorbent assay (ELISA) β And IL-18. Results: Compared with those in the control group, the cell activity of LPS group was significantly decreased, the apoptotic index increased significantly, the protein expression of NLRP3, caspase-1 and GSDMD-C, and the contents of IL-1β and IL-18 were significantly increased (all P<0.05); compared with those in LPS group, the cell activity of Dahuang Mudan Decoction group was significantly increased, the apoptotic index decreased significantly, the protein expression of NLRP3, caspase-1 and GSDMD-C, and the contents of IL-1β and IL-18 were significantly decreased (all P<0.05). Conclusion: Dahuang Mudan Decoction can inhibit NLRP3/caspase-1 pathway and reduce LPS-induced pyrosis of intestinal epithelial cells.
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2021, Vol. 27 
