miR-135b和LZTS1 mRNA HE4 mRNA在卵巢癌中的表达及临床意义

doi:10.3969/j.issn.1006-6233.2021.09.011

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Abstract Objective: To observe the expression level and clinical significance of microRNA-135b (miR-135b), leucine zipper tumor suppressor gene 1 (LZTS1) messenger RNA (mRNA), human epididymal protein 4 (HE4) mRNA in ovarian cancer. Methods: 56 patients with ovarian cancer in our hospital from January 2018 to November 2020 were selected, and the ovarian cancer tissues and their corresponding adjacent tissues were collected. The expression levels of miR-135b, LZTS1 mRNA and HE4 mRNA in ovarian tissues of the two groups were detected by real time fluorescent quantitative PCR; the relationships between the expressions of miR-135b, LZTS1 mRNA, HE4 mRNA and the clinicopathological features of ovarian cancer were analyzed; and the diagnostic values of expression levels of miR-135b, LZTS1 mRNA and HE4 mRNA in ovarian cancer were analyzed. Results: The expression levels of miR-135b and HE4 mRNA in ovarian cancer group were significantly higher than those in adjacent group (P<0.05), and the expression level of LZTS1 mRNA was significantly lower than that in adjacent group (P<0.05); the expression levels of miR-135b, LZTS1 mRNA and HE4 mRNA in ovarian cancer were correlated with tumor differentiation, TNM stage and lymph node metastasis (P<0.05); the area under curve (AUC) of miR-135b, LZTS1 mRNA and HE4 mRNA in the diagnosis of ovarian cancer were 0.781, 0.755 and 0.773, respectively, the corresponding cutoff values were 1.25, 0.87 and 1.20, respectively, at this time, the corresponding sensitivities were 74.5%, 76.4% and 72.7%, and the specificities were 77.2%, 72.9% and 77.2%, respectively; the AUC of the combined diagnosis of ovarian cancer was 0.943, and the sensitivity and specificity were 90.9% and 84.2% respectively. Conclusions: MiR-135b and HE4 mRNA are highly expressed in ovarian cancer, LZTS1 mRNA is lowly expressed, miR-135b, LZTS1 mRNA and HE4 mRNA have certain diagnostic value for ovarian cancer, and the combination of them can effectively improve the diagnostic efficacy for ovarian cancer.

Key words： microRNA-135b Leucine zipper tumor suppressor 1 Human epididymal protein 4 Ovarian cancer

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.09.011 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I9/1454

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