|
|
|
| Levels and Clinical Significance of Peripheral Blood Regulatory T Lymphocytes IL-10 and SIgE in Children with Bronchial Asthma |
| WANG Ruqiong, LI Xiaoming, LIN Daojiong, et al |
| Hainan Women's and Children's Medical Center, Hainan Haikou 570000, China |
|
|
|
|
Abstract Objective: To explore the clinical guiding significance through detecting the levels of peripheral blood regulatory T lymphocytes, serum interleukin-10 (IL-10) and specific IgE (sIgE) in bronchial asthma children allergic to Dermatophagoides pteronyssinus. Methods: From February 2018 to February 2020, 163 children with bronchial asthma who were allergic to Dermatophagoides pteronyssinus were selected as study subjects. According to the severity of the disease, they were divided into mild group (52 cases), moderate group (63 cases), and severe group (48 cases). Meanwhile, 50 healthy children of the same age were selected as the control group. The peripheral blood lymphocytes were isolated, and then cultured for 48 hours under the stimulation of Dermatophagoides pteronyssinus leachate. Flow cytometry was used to detect the percentages of adaptive regulatory T cells (iTreg) and type I regulatory T cells (Tr1). In addition, the lung function, serum IL-10, TGF-β and sIgE levels were compared among groups. Results: Comparison of the control group and the diseased group found that the severer the disease, the lower the forced expiratory volume in the first second (FEV1), the FEV1 to forced vital capacity (FVC) ratio (FEV1/FVC) and peak expiratory flow (PEF), the lower the proportions of iTreg and Tr1, showing statistically significant differences (P<0.05). Comparison of immune factor levels between the control group and the diseased group found that the severer the disease, the lower IL-10 and TGF-β levels (P<0.05), and the higher the sIgE level (P<0.05). Conclusion: The detection of lung function indexes, IL-10 secreting regulatory T lymphocytes and immune factors IL-10, TGF - β and SiGe levels in children with bronchial asthma not only helps to evaluate the severity of the disease, but also helps to provide reference for the prognosis of the disease in the process of treatment..
|
|
|
|
|
|
[1] Bonaventure A,Orsi L,Rudant J,et al.Genetic polymorphisms of Th2 interleukins,history of asthma or eczema and childhood acute lymphoid leukaemia: Findings from the ESCALE study (SFCE)[J].Cancer Epidemiol,2018,55:96~103.
[2] 滑丽芳,王鸣飞.哮喘患儿T淋巴细胞亚群水平变化及意义[J].宁夏医科大学学报,2018,40(4):474~476.
[3] 张义,杨勤,靳蓉,等.特异性免疫治疗对支气管哮喘患儿血清调节性T淋巴细胞水平、IL-10、sIgE水平的影响及疗效评价[J].贵州医药,2020,44(10):1525~1528.
[4] Hong S W,Eunju O,Lee J Y,et al.Interleukin-2/antibody complex expanding Foxp3 + regulatory T cells exacerbates Th2-mediated allergic airway inflammation[J].BMB Rep,2019,52(4):283~288.
[5] 中华医学会儿科分会呼吸学组.儿童支气管哮喘诊断与防治指南(五)(2008年修订)[J].中华医学信息导报,2009,24(5):20~21.
[6] 杨丹,刘碧翠,刘春涛.Ⅱ型固有淋巴细胞及其在支气管哮喘中的作用[J].中华临床免疫和变态反应杂志,2018,12(1):74~80.
[7] Rui S,Zhaohua L,Yanyan W,et al.Imbalance between Th17 and regulatory T cells in patients with systemic lupus erythematosus combined EBV/CMV viraemia[J].Clin Exp Rheumatol,2020,38(5):864~873.
[8] 王彩虹,潘晓娴,陈金梅,等.调节性T细胞在小鼠放射性肺纤维化进程中的动态变化[J].江苏大学学报(医学版),2020,30(1):34~38,44.
[9] 范广来,徐妍,马希贵,等.哮喘患儿血清IFN-γ,IL-4,IL-10水平及与肺功能的关系[J].中国现代医学杂志,2020,30(7):48~52. |
|
|
|
2021, Vol. 27 
