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| Silk Fibroin/Hydroxyapatite Composite Promotes Bone Defect Repair by Inhibiting NF - κ B / NLRP3 |
| LI Shiyan, LI Yanbo, CAO Lei, et al |
| Chengde Central Hospital, Hebei Chengde 067000, China |
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Abstract Objective: To study the effect of silk fibroin hydroxyapatite (HA) composite on inflammatory reaction in repairing rabbit bone defect. Methods: A total of 16 New Zealand white rabbits were divided into two groups. 15mm long radial segmental bone defect model was made in the operation groups. According to the different transplantation materials, the rabbits were divided into two groups. One group was implanted with HA, the other group was implanted with SF surface modified HA. The mRNA expression of IL-1 β and IL-18 were detected by real-time fluorescent quantitative polymerase chain reaction (RT PCR) 48 hours after operation. The protein expression levels of NF - κ B and NLRP3 were detected by Western blot. Results: Compared with the tissue-engineered bone prepared by HA culture with SF surface modification, the mRNA levels of IL-1 β and IL-18, as well as the protein expression levels of NF-κB and NLRP3 were significantly decreased. Conclusion: Silk fibroin hydroxyapatite composite can promote bone defect repair by inhibiting NF-κB/NLRP3 mediated inflammatory reaction.
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2021, Vol. 27 
