MicroRNA-216b靶向DCLK1调控卵巢癌HO-8910细胞的增殖迁移和侵袭

doi:10.3969/j.issn.1006-6233.2021.04.02

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Abstract Objective: To investigate the effect of MicroRNA-216b (miR-216b) on the proliferation, migration and invasion of ovarian cancer HO-8910 cells.Methods: The expression of miR-216b in cancer and paracancerous tissues of 30 patients with ovarian cancer was detected by qPCR. Experimental cells were divided into four groups: control group (normal HO-8910 cells), negative control group (transfected empty plasmid HO-8910 cells), overexpression group (transfected miR-216bmimicHO-8910 cells), low expression group (transfected miR-216binhibitorHO-8910 cells). CCK8 assay, scratch test, plate cloning and Transwell were used to detect the ability of cell proliferation, migration and invasion. Westernblot and qPCR were used to detect the expression of biscorticoid kinase 1 (Doublecortinlikekinase1, DCLK1) protein and mRNA. Results: The expression of miR-216b in ovarian cancer tissues was significantly lower than that in paracancerous tissues, and the results of CCK8 assay, scratch test and Transwell showed that the ability of cell proliferation, migration and invasion in miR-216bmimic group was significantly lower than that in control group and negative control group, while that in miR-216binhibitor group was significantly higher than that in control group and negative control group. The results of Western and qPCR showed that the expression levels of DCLK1 protein and mRNA in miR-216bmimic group were significantly lower than those in control group and negative control group, while those in miR-216binhibitor group were significantly higher than those in control group and negative control group. Conclusion: MiR-216b can target DCLK1 to inhibit the proliferation, migration and invasion of ovarian cancer HO-8910 cells. MiR-216b may be a new target for diagnosis and treatment of ovarian cancer.

Key words： miR-216b Dicorticoid kinase 1 Ovarian cancer HO-8910 cells Proliferation

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	FAN Tao
	LI Jiangpeng

Cite this article:

FAN Tao,LI Jiangpeng. MicroRNA-216b Targets DCLK1 Regulating the Proliferation, Migration and Invasion of Ovarian Cancer HO-8910 Cells[J]. HeBei Med, 2021, 27(4): 534-538.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.04.02 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I4/534

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