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Effect of LPA on Biological Characteristics of Cervical Cancer Stem Cells and Regulation of RhoA/Rock2 Pathway |
ZHOU Yong, LUO Baoping, et al |
Hubei Hospital of Traditional Chinese Medicine, Hubei Wuhan 430060, China |
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Abstract Objective: To study and explore the effects of lysophosphatidic acid (LPA) on the biological characteristics of cervical cancer stem cells and the regulation of RhoA/Rock2 pathway, further clarify the pathogenesis of cervical cancer and develop cervical cancer targets for the future. Point drugs provide theoretical basis. Methods: Siha cell lines were used as primary cells. The cervical cancer stem cells were cultured with balloon-encapsulated cells to observe the ability of cell colony formation. Cell surface markers were detected by FACS. After the successful cultivation of cervical cancer stem cells, they were divided into control group, LPA group and inhibition group. The control group was cultured normally. In the LPA group, LPA of different concentrations (1, 5, 10, 15, 20 umol/L) was added, and the inhibitor group was The optimal proliferative concentration of LPA cells group was based on the addition of Rho kinase inhibitor Y-27632. Cell proliferation was observed and cell growth curves were plotted. The mRNA levels of LPA, RhoA and Rock2 in each group were detected by PCR. The expression of RhoA and Rock2 was detected. Results: After culture, there were suspension cell spheres with CD44+, CK17+, CD34- and CD105- phenotypes. Cervical cancer stem cells were successfully obtained. After adding LPA, the proliferation of cancer cells was significantly promoted, and there was a time In a dose-dependent manner (P<0.05), addition of Y-27632 significantly inhibited cell proliferation (P<0.05). LPA, RhoA, and Rock2 mRNA levels and western blot were significantly higher in LPA group than in the control group and inhibition. Group (P<0.05). Conclusion: LPA can promote the proliferation of cervical cancer stem cells, and its mechanism of action can be achieved by regulating the RhoA/Rock2 pathway.
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