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| Effect of Serum Containing Yuzhi Zhixue Granule on PTEN/PI3K/AKT Signal Pathway in Ectopic Endometrial Stromal Cells |
| LIU Yanjie, et al |
| The First Affiliated Hospital of Zhengzhou University, Henan Zhengzhou 450000, China |
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Abstract Objective: To investigate the effect of serum containing Yuzhi Zhixue granule on the signal pathway of phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidyl inositol-3-kinase (PI3K)/protein kinase B (AKT) in ectopic endometrial stromal cells (ESCs). Methods: The serum containing Yuzhi Zhixue granule was prepared, and human ectopic ESCs cells were cultured in vitro, which were divided into control group (blank serum), low dose group (low dose Yuzhi Zhixue granules serum), middle dose group (middle dose Yuzhi Zhixue granules serum) and high dose group (high dose Yuzhi Zhixue granules serum). CCK-8 method was used to detect cell proliferation; cell invasion was detected by Transwell method; the expression of PTEN mRNA was detected by real-time fluorescent quantitative PCR (qRT-PCR); and the expressions of PTEN, p-PI3K, PI3K, p-AKT and AKT proteins were detected by Western blot (WB). Results: Compared with those in the control group, the OD value, invasive cell number, p-PI3K/PI3K, p-AKT/AKT proteins expression levels of ectopic ESCs cells in the low, middle and high dose groups were significantly lower (P<0.05), and the expressions of PTEN mRNA and protein were significantly higher (P<0.05); at the same time, with the increase of dosage of serum containing Yuzhi Zhixue granule, the OD value, invasive cell number, p-PI3K/PI3K, p-AKT/AKT protein expression level of ectopic ESCs cells decreased gradually (P<0.05), and the expressions of PTEN mRNA and protein increased gradually (P<0.05). Conclusion: Serum containing Yuzhi Zhixue granule can inhibit the proliferation and invasion of ectopic ESCs cells, up-regulate the PTEN expression and inhibit PI3K/AKT signaling pathway.
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2021, Vol. 27 
