AmpC ompK35在亚胺培南诱导肺炎克雷伯菌耐药中的相关性研究

doi:10.3969/j.issn.1006-6233.2021.01.025

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Abstract Objective: To investigate the role of AmpC and ompk35 in imipenem induced resistance of Klebsiella pneumoniae. Methods: A total of 200 strains of Klebsiella pneumoniae isolated and cultured in our hospital from January 2015 to January 2020 were collected, among which 50 strains were resistant to imipenem (group A), 150 strains were not resistant to imipenem (group B), the gene expression of AmpC and ompk35 was detected by PCR, and the protein expression of AmpC and ompk35 was detected by Western blot. Results: The relative expression of AmpC gene and protein in group A were (1.658±0.122) and (1.501±0.106), which were significantly higher than that in group B (P < 0.05); The relative expression of ompk35 gene and protein in group A were (0.712±0.110) and (0.611±0.107), which were significantly lower than that in group B (P < 0.05); The correlation between AmpC gene and ompk35 gene was negative (r=-0.329, P < 0.05); the correlation between AmpC protein and ompk35 protein was negative (r=-0.435,p＜0.05). Conclusion: The expression of AmpC in imipenem resistant Klebsiella pneumoniae increase, and the expression of ompk35 decrease. They may play an important role in the mechanism of drug resistance.

Key words： AmpC Ompk35 Imipenem Klebsiella pneumoniae

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.01.025 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I1/109

[1] 钟太清,李毓龙,刘宝涛,等.医院获得性血流感染来源碳青霉烯类耐药肺炎克雷伯菌耐药分析[J].中国实验诊断学,2018,22(5):765~768.
[2] 周宜庆,阮永春,周洁,等.肺炎克雷伯菌Hfq蛋白特性及对耐药的影响[J].微生物与感染,2020,15(2):88~97.
[3] 许光辉,俞柳敏,陈淑娟,等.膜孔蛋白基因表达与耐碳青霉烯类肺炎克雷伯菌耐药性关系的研究[J].检验医学与临床,2018,15(16):2405~2408,2411.
[4] 孔子艳,沈俊,李心愿,等.患儿分离的碳青霉烯类耐药肺炎克雷伯菌耐药基因分析[J].中华医院感染学杂志,2018,28(20):3124~3127,3131.
[5] 安翔宇,谢伟峰,郑旭,等.青岛市区5家医院耐碳青霉烯类肺炎克雷伯杆菌耐药基因检测及分析[J].中华危重病急救医学,2020,32(2):145~149.
[6] Gomes Ana Erika InAcio,Prado S L,Siqueira Nathalia Maria Gonalves,et al. Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR[J]. Scientific Reports,2018,8(1):9001.
[7] Margaret M C L,Wyres K L,Duchêne Sebastian,et al. Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination[J]. Nature Communications,2018,9(1):2703.
[8] Ghaffarian F,Hedayati M,Ebrahim-Saraie H S,et al. Molecular epidemiology of ESBL-producing klebsiella pneumoniae isolates in intensive care units of a tertiary care hospital,North of Iran[J]. Cellular and Molecular Biology,2018,64(7):75~79.
[9] NK Tulara. Nitrofurantoin and fosfomycin for extended spectrum beta-lactamases producing escherichia coli and klebsiella pneumoniae.[J]. Journal of Global Infectious Diseases,2018,10(1):19~21.
[10] Kim SJ,Chu ST,Lee KS,et al. Metastatic endophthalmitis and thyroid abscess complicatingKlebsiella pneumoniaeliver abscess[J]. Clinical & Molecular Hepatology,2018,24(1):88~91.
[11] 何晓静,李晓冰,于莹,等.肺炎克雷伯菌对左氧氟沙星耐药的分子机制[J].中国医院药学杂志,2018,38(15):17~20.
[12] 杨雪,刘琳,赵丹,等.耐碳青霉烯类肺炎克雷伯菌耐药基因检测与分子流行病学研究[J].重庆医学,2018,47(15):1977~1980,1985.
[13] 黄峰,许元元.耐碳青霉烯类肺炎克雷伯菌耐药基因检测及同源性[J].中国感染控制杂志,2018,17(1):21~25.
[14] 苏乐斌,李柏生,谭海芳,等.广东省肇庆市肺炎克雷伯菌临床分离株的耐药性与MLST分型研究[J].中国抗生素杂志,2019,44(2):112~117.