CIAPIN1通过抑制ERK1/2途径对膀胱尿路上皮癌增殖和转移的作用研究

doi:10.3969/j.issn.1006-6233.2021.01.012

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Abstract Objective: To explore the role and possible mechanism of CIAPIN1 in bladder urothelial carcinoma. Methods: Bladder urothelial cancer cell lines J82, T24, UM-UC-3 and human bladder epithelial immortalized cells SV-HUC-1 were used as the research objects. qRT-PCR was used to detect the expression level of CIAPIN1 mRNA in the cells; Western blot was used to detect the expression levels of CIAPIN1 p-ERK1/2 and ERK1/2 protein in cells; 5-ethynyl-2'-deoxyuridine (EdU) labeling test was used to detect cell proliferation; cell scratch test was used to detect cell migration; Transwell test was used to detect cell invasion. Results: Compared with SV-HUC-1 group, the expression levels of CIAPIN1 mRNA and protein in cells of T24, UM-UC-3 and J82 groups were significantly increased (P<0.05). Cells in T24 group had the most obvious changes, so T24 cells were selected for subsequent experiments. Compared with the NC group, the number of EdU-positive cells, cell migration distance, and invasive cells in the si-CIAPIN1 group were significantly reduced (P<0.05), and protein expression level of p-ERK1/2/ERK1/2 was significantly increased (P<0.05) ; Compared with the si-CIAPIN1 group, the number of EdU positive cells, migration distance, and number of invasive cells in the si-CIAPIN1+ U0126 group were significantly increased (P<0.05), and the protein expression level of p-ERK1/2 / ERK1/2 was significantly reduced ( P<0.05). Conclusion: CIAPIN1 promotes the proliferation and metastasis of bladder urothelial cancer cells by inhibiting ERK1/2 pathway phosphorylation.

Key words： CIAPIN1 Bladder urothelial carcinoma Proliferation Metastasis ERK1/2 pathway

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.01.012 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I1/50

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