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| The Relationship between MICA Gene Expression of Bladder Cancer and Mutation of p53 and K-ras Gene |
| GU Jingfeng, WANG Guiqi, et al |
| The First Hospital of Hebei Medical University, Hebei Shijiazhuang 050031, China |
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Abstract Objective: To investigate the relationship between the expression of MICA gene in bladder cancer and the mutation of p53 gene and K-ras gene. Methods: 66 cases of bladder cancer patients with clinical data in our hospital from March 2014 to March 2016 were analyzed. Semi quantitative PCR-SSCP technique was used to detect the expression of MDR1 gene and p53 and K-ras in cancer tissues, and analyzed the relationship between the expression of MICA gene in colorectal cancer and the mutation of p53 and K-ras genes. Results: The study found that there was no correlation between the expression of MICA and the location, age and sex of the patients with colorectal cancer (P > 0.05). In 66 patients, the mean expression of MICA in 22 lymph node metastasis patients was higher than that without lymph node metastasis, and the difference was statistically significant (P < 0.05). 8 cases of K-ras and p53 gene expression of the MICA gene mutation combined with average weight is higher than non mutation, the difference was statistically significant (P < 0.05), 12 cases of K-ras gene mutation MICA average expression was slightly higher than that without mutation, but the difference was not statistically significant (P > 0.05), 16 cases of p53 gene mutation MICA expression was significantly better than the average mutation in the p53 gene, the difference was statistically significant (P < 0.05). Conclusion: The high expression of MICA gene has become a key indicator for the detection of bladder cancer, and the correlation between K-ras and p53 gene and its high expression.
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[1] 陈曦.中国人结直肠癌中APC、p53、K-ras和PIK3CA基因突变的研究[D].安徽医科大学,2012.
[2] 马列,赵明静,王群,李秀林,王笑歌.结直肠癌p53、FHIT、K-RAS基因突变与吸烟相关性Meta分析[J].现代肿瘤医学,2011,26(10):1982~1987.
[3] 李婷婷.HOXB7激活WnT/β-Catenin信号通路促进结直肠癌侵袭转移的分子机制[D].南方医科大学,2013.
[4] 赵莉.结直肠癌临床特点及癌变相关分子机制的异同分析[D].北京协和医学院,2010.
[5] Lteif A A,Han K,Mather KJ.Obesity,insulin resistance and the metabolic syndrome:determinants of endothelial dysfunction in whites and blacks[J].Circulation,2013,112(1):32~38.
[6] Harred J F,Knight AR, McIntyre JS.Dow chemical campany, assignee expoxidation process[J].USP atent 3,2012,3(17):1927~1904.
[7] Foley RN,Parfrey PS,Sarnak MJ.Epidemiology of cardiovasc-ular disease in chronic renal disease[J].Am Soc Nephrol,2013,9 (12Suppl):16~23.
[8] Malyszko J.Mechanism of endothelial dysfunction in chronic kidney disease[J].Clin Chim Acta,2010,411(19/20):1412~1420.
[9] Izumi S,Muano T,Mori A,et al.Common carotid artery stiffness cardiovascular function and lipid metabolism after menopause[J].Life Sci,2012,78(15):1696~1701. |
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2017, Vol. 23 
