miR-23b和notch1在瘢痕疙瘩中的表达关系研究

doi:10.3969/j.issn.1006-6233.2020.12.019

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Abstract Objective: To detect the expressions of microRNA-23b (miR-23b) and notch1 protein in keloid fibroblasts, and to explore the relationship between the expressions and their effects on keloid. Methods: From December 2017 to November 2019, 130 patients with keloid were collected from the 149 Hospital of the PLA. During the operation, the scar tissues and normal dermal tissues of each patient were taken at the same time. The scar size was recorded, According to the severity of keloid, it was divided into mild 65 cases, moderate 43 cases and severe 22 cases. Firstly, fibroblasts from keloid and normal dermis were extracted and cultured, the level of miR-23b in fibroblasts was detected by real-time fluorescent quantitative PCR (qRT-PCR), and the level of notch1 protein was detected by immunohistochemistry. Pearson method was used to evaluate the correlation between miR-23b and notch1 expressions in keloid. Results: There were 203 skin lesions in 130 patients with keloids, 78 on the chest (38.42%), 59 on the shoulder and back (29.07%), 40 on the limbs (19.71%), 13 on the ears (6.40%), and 8 on the abdomen (3.94%), 5 on the hip (2.46%). The cumulative area of skin lesions was 6 cases (4.61%) with large scars, 57 cases (43.85%) with medium scars, and 67 cases (51.54%) with small scars. Compared with normal dermal tissues, mir-23b mRNA expression level and Notch1 positive expression rate in fibroblasts in keloid tissues were significantly increased, with statistically significant differences (66.15% vs 40.00 %, P<0.05). The expressions of miR-23b mRNA and notch1 in different clinical grades of keloid were statistically significant (P<0.05), and with the aggravation of keloid, miR-23b mRNA level and notch1 positive expression rate increased; Pearson correlation analysis showed that miR-23b and Notch-1 were positively correlated in keloid tissue fibroblasts (r = 0.529, P<0.05). Conclusions: MiR-23b mRNA and Notch-1 protein are highly expressed in keloid tissue, and they are positively correlated, which may be involved in the formation and development of keloid at the same time.

Key words： MicroRNA-23b Notch1 protein Keloid Expression Correlation

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HE Xuedong,et al. Research on the Expression Relationship of MiR-23b and Notch1 in Keloid[J]. HeBei Med, 2020, 26(12): 2016-2020.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.12.019 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I12/2016

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