萝卜硫素通过Nrf2/ARE信号通路对脂多糖性急性肺损伤机制的保护作用

doi:10.3969/j.issn.1006-6233.2020.12.06

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Abstract Objective: To study whether sulforaphane (SF) can reduce the acute lung injury (ALI) by up-regulating the transcription factor NF-E2 related factor 2 (Nrf2) to play an antioxidant and anti-inflammatory role. Methods: Thirty-two healthy mice were randomly divided into acute lung injury group (ALI group), control group (control group), SF group, and SF solvent group, 8 mice in each group. The control group was injected intraperitoneally with 10 mg/kg saline. SF group was intraperitoneally injected with 20 mg/kg (SF), SF solvent group was intraperitoneally injected with 20 mg/kg (SF carrier) solvent, 2 twice a day, continuous injection was performed for 3 days, and ALI group, SF group, and SF solvent group were injected with lipopolysaccharide at the 4th day. (LPS) 10 mg/kg, and an endotoxin-induced ALI model was prepared. Six hours after injection of LPS or normal saline, vena cava blood was removed and serum IL-6 and TNF-ɑ levels were measured by ELISA. Pulmonary tissue pathological results were scored for lung injury after observation with light microscope. Nrf2 nucleus in mouse lung tissue Protein expression was measured by Western blotting, and lung dry weight/wet weight ratio was calculated simultaneously. Colorimetric method was used to detect SOD, MPO and iNOS content in lung tissue homogenate. Results: The lung injury score, iNOS, MPO, IL-6, and TNF-ɑ levels in the ALI group and the SF solvent group were significantly higher than those in the control group, and the expression of Nrf2 protein was up-regulated (P<0.05). SOD was lower than that in the control group (P<0.05). There was no significant difference in the above indicators between the ALI group and the SF solvent group (P>0.05). The IL-6, TNF-ɑ, lung injury score, and iNOS content in the SF group were significant. Compared with the ALI group, the lung dry weight/wet weight ratio and SOD content increased, and the expression of Nrf2 protein was up-regulated (P<0.05). Conclusion: SF can reduce the severity of LPS-induced acute lung injury by inducing the expression of Nrf2 protein through SF, and it can also reduce the levels of IL-6, TNF-ɑ and MPO, confirming that Nrf2 transcription factor can effectively protect ALI.

Key words： Acute lung injury Sulforaphane Nrf2 Oxidative stress

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.12.06 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I12/1961

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