颅脑损伤患儿血清中miR-34a和NO TNF-α表达及意义

doi:10.3969/j.issn.1006-6233.2020.12.03

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Abstract Objective: To study the expression levels of microRNA-34a (miR-34a), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in serum of children with craniocerebral injury and their significance. Methods: 78 children with craniocerebral injury admitted to Qinghai Women and Children's Hospital from September 2017 to August 2019 were divided into mild group (23 cases), moderate group (30 cases) and severe group (25 cases) according to Glasgow Coma score(GCS); at the same time, 80 healthy controls in the same period were selected. Real-time quantitative PCR (qRT-PCR) was used to detect the level of miR-34a in serum; the level of NO in serum was detected by chemical colorimetry; and enzyme-linked immunosorbent assay (ELISA) was used to detect the level of TNF-α in serum. Pearson method was used to analyze the correlation between serum miR-34a, NO, TNF-α and GCS score. Receiver operating characteristic curve (ROC) was applied to analyze the predictive value of serum miR-34a, NO, TNF-α levels for TBI. Results: ①The levels of serum miR-34a, NO and TNF-α in children with craniocerebral injury were higher than those in the control group, and the differences were significant (P<0.05); ②The levels of serum miR-34a, NO and TNF-α in severe group were higher than those in moderate group and mild group, the levels of serum miR-34a, NO and TNF-α in moderate group were higher than those in mild group, and the differences were significant (P<0.05); ③Pearson analysis showed that levels of serum miR-34a, NO and TNF-α were negatively correlated with GCS scores in children with craniocerebral injury (P<0.05); ④The area under the curve of TBI predicted by serum miR-34a, NO and TNF-α levels is 0.821(95%CI:0.675~0.967), 0.902(95%CI:0.816~0.989), 0.851(95%CI:0.741~0.961); cutoff values are 0.65, 38.45 μmol/L, 153.49 pg/mL; sensitivity is 88.9%,94.4%,72.2%; specificity is 72.0%, 70.0%, 98.0%; The area under the curve predicted by the joint detection is 0.998 (95%CI: 0.992~1.000); the sensitivity is 100.0%; and the specificity is 98.0%; ⑤The levels of serum miR-34a, NO and TNF-α in the death group were significantly higher than those in the survival group, and the differences were significant (P<0.05). Conclusions: miR-34a, NO, and TNF-α are all highly expressed in the serum of children with craniocerebral injury, which is closely related to the degree of craniocerebral injury. Joint detection has certain predictive value, and it has a certain degree of assessment on the grim prognosis of children with craniocerebral injury.

Key words： Craniocerebral injury MicroRNA-34a Nitric oxide Tumor necrosis factor-α

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	WANG Shengyi
	SUN Xiaohong
	et al

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WANG Shengyi,SUN Xiaohong,et al. Expressions and Significance of Serum MiR-34a NO and TNF-α in Children with Craniocerebral Injury[J]. HeBei Med, 2020, 26(12): 1947-1951.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.12.03 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I12/1947

[1] Kochar A, Borland ML, Phillips N, et al. Association of clinically important traumatic brain injury and Glasgow Coma Scale scores in children with head injury[J]. Emerg Med, 2020,37(3):127~134.
[2] Ge X, Gao J, Sun QW, et al. MiR-34a inhibits the proliferation, migration, and invasion of oral squamous cell carcinoma by directly targeting SATB2[J].Cell Physiol, 2020, 235(5):4856~4864.
[3] Wang SP, Wang D, Li HX, et al. Influence of miR-34a on cerebral neuronal apoptosis in rats with cerebral ischemia reperfusion through the Notch1 signaling pathway[J]. Eur Rev Med Pharmaco, 2019,23(18):8049~8057.
[4] 赵志茹,赵雄飞,徐家萍,等.盐酸贝那普利对老年痴呆患者血清及脑脊液中一氧化氮乙酰胆碱酯酶水平的影响[J].山西医药杂志,2019,48(4):457~460.
[5] 曾旦丹,赵迎春,朱莹莹,等.新生儿缺氧缺血性脑病血清UA、CysC及TNF-α水平变化及与脑损伤的相关性研究[J].现代生物医学进展,2018,18(14):2665~2668.
[6] Samuel JS. Glasgow coma scale score in trauma triage: a measurement without meaning[J]. Ann Emerg Med, 2018, 72(3):270~271.
[7] Salley J, Crook L, Ciccia A, et al. Traumatic brain injury in young children: a scoping review[J]. Semin Speech Lang, 2020, 41(2):125~142.
[8] Abecasis F, Cardim D, Czosnyka M, et al. Transcranial doppler as a non-invasive method to estimate cerebral perfusion pressure in children with severe traumatic brain injury[J]. Child Nerv Syst, 2020, 36(1):125~131.
[9] Wang B, Li D, Kovalchuk I, et al. miR-34a directly targets tRNAiMet precursors and affects cellular proliferation, cell cycle and apoptosis[J]. P Natl Acad Sci USA, 2018, 115(28):7392~7397.
[10] Tolosa E, Botta-Orfila T, Morató X, et al. MicroRNA alterations in iPSC-derived dopaminergic neurons from parkinson disease patients[J]. Neurobiol Aging, 2018, 69(1):283~291.
[11] Pan X, Shao Y, Wang F, et al. Protective effect of apigenin magnesium complex on H₂O₂-induced oxidative stress and inflammatory responses in rat hepatic stellate cells[J]. Pharm Biol, 2020, 58(1):553~560.
[12] 吕学文,杨永康,张小雷,等.创伤性颅脑损伤患者凝血功能及炎性因子的变化研究[J].临床外科杂志,2019,27(3):247~249.
[13] 张连钰,白焕英,施镔,等.不同严重程度急性颅脑损伤患者炎症指标差异性对比[J].医学综述,2019,25(23):4776~4780.
[14] Massaro AN, Wu YW, Bammler TK, et al. Dried blood spot compared to plasma measurements of blood-based biomarkers of brain injury in neonatal encephalopathy[J]. Pediatr Res, 2019, 85(5):655~661.
[15] 王娜,刘春丽,邓爱平,等.颅脑损伤患者高凝血状态与炎性因子水平和NF-κB活性关系分析[J].疑难病杂志,2018,17(10):1117~1121,1189.