草乌甲素通过调节TRPV1受体功能减轻神经病理性疼痛大鼠疼痛的机制

doi:10.3969/j.issn.1006-6233.2020.11.02

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Abstract Objective: To investigate mechanisms of bulleyaconitine A relieving pain in rats with neuropathic pain by regulating the transient receptor potential vanilloid type 1(TRPV1) receptor function.Methods: 100 SD rats were divided into control group,model group,dexamethasone group,low and high dosage groups of bulleyaconitine A;model group,dexamethasone group,low and high dosage groups of bulleyaconitine A to establish neuropathic pain model.The rats in the dexamethasone group (40mg/kg) and the low (40mg/kg) and high (80mg/kg) dosage groups of bulleyaconitine A were given intrathecal injection (Injection volume:1.0mL/100g) on the first day of modeling and continuously for 3 weeks.The control group and the model group received intrathecal injection for the same volume of physiology Brine.At the end of the experiment,the mechanical pain threshold,thermal pain threshold,and pain-weighted score were determined.The expression of transient receptor potential vanilloid type 1 (TRPV1) mRNA and protein in spinal cord tissue was determined by real-time fluorescence reverse transcription and immunohistochemistry.Determination of Fas,Caspase-3 and Caspase-6 in spinal cord tissue by immunosorbent assay.Results: Compared with the control group,the mechanical pain threshold and thermal pain threshold of the model group were decreased in the model group,and PIS score,TRPV1 mRNA and protein,Fas,Caspase-3,and Caspase-6 protein levels were increased (P<0.05).Compared with the model group,the mechanical pain threshold,thermal pain threshold increased,PIS score,TRPV1 mRNA and protein,Fas,Caspase-3,Caspase-6 protein levels were decreased in the dexamethasone group and low and high dosage groups of bulleyaconitine A,and the levels of PASP were decreased (P<0.05).The mechanical pain threshold and thermal pain threshold were higher in the high-dose group than those in the low-dose group (P<0.05).The PIS score,TRPV1 mRNA and protein,Fas,Caspase-3,and Caspase-6 protein levels were lower than those in the high-dose group than in the low-dose group (P<0.05);Compared with dexamethasone group,the mechanical pain threshold and thermal pain threshold were lower in the low and high dosage groups of bulleyaconitine A (P<0.05),PIS score,TRPV1 mRNA and protein,Fas,Caspase-3,Caspase-6 protein levels increased (P<0.05).Conclusion: The mechanism is related to the inhibition of TRPV1 receptor function and neuronal apoptosis of spinal cord by bulleyaconitine A,which can alleviate neuropathic pain in rats.

Key words： Bulleyaconitine A TRPV1 Neuropathic pain

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.11.02 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I11/1765

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