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| Effects of Anti-bronchial Syrup Combined with Glucocorticoid on NF-κB Signaling Pathway in Model Rats with Cough Variant Asthma |
| GUO Xin, ZHUGE Shurui, QI Xusheng |
| Shiyan Taihe Hospital / Affiliated Hospital of Hubei Medical College, Hubei Shiyan 442000, China |
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Abstract Objective: To study the effects of anti-bronchial syrup combined with glucocorticoid on NF-κB signaling pathway in model rats with cough variant asthma. Methods: 50 SD rats were selected and randomly divided into control group, model group, anti-bronchial syrup group, glucocorticoid group and combined group, with 10 rats in each group. On the 1st day of the experiment, the other four groups except control group were injected intraperitoneally with ovalbumin (OVA) and aluminum hydroxide, and they were treated for three times with once/7days for rat asthma model construction. On the 21st day of the experiment, they were given ovalbumin inhalation for atomization stimulation, and control group was stimulated with normal saline for 3 times/week with 30 min/time. Each drug-administered group was pretreated with drugs at 30 min before atomization stimulation, and control group was treated with the same amount of normal saline. 3 weeks after stimulation, HE staining was used to observe the pathological changes of the lung tissue, and the thickness of airway wall and the thickness of smooth muscle were measured. Western blot was used to detect the expression levels of IκB, NF-κB, MMP-9 and VEGF proteins in rat lung tissue. Results: HE staining showed that combined group could significantly improve the airway wall epithelial tissue necrosis and shedding, and a large number of inflammatory cell infiltration. The analysis of airway wall and smooth muscle thickening showed that the airway wall and smooth muscle thickening in the other four groups were significantly thickened compared with control group (P<0.05). Compared with model group, the degree of tissue thickening in drug-treated groups was significantly decreased, and the degree in combined group was significantly lower than that in single drug groups (P<0.05). Compared with control group, the expression level of IκB protein was significantly decreased in the other four groups, and the expression levels of NF-KB, MMP-9 and VEGF were significantly increased (P<0.05). Compared with model group, the expression level of IKB protein in single drug groups and combined group was significantly increased, and the expression levels of NF-KB, MMP-9 and VEGF were significantly decreased (P<0.05), and the changes of protein expression in combined group were significantly higher than those in single drug groups (P<0.05). Conclusion: Kangzhi syrup combined with dexamethasone can reduce the hydrolysis of I κ B protein, decrease the release of NF-κ B protein, down regulate the expression of MMP-9 and VEGF, and inhibit the airway remodeling of cough variant asthma rats.
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2020, Vol. 26 
