Beclin1调控的自噬在胚胎碎片形成中的作用及机制研究

doi:10.3969/j.issn.1006-6233.2020.10.004

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Abstract Objective: To study the role and possible mechanism of beclin1-regulated autophagy in embryo fragment formation. Methods: A total of 240 patients who underwent in vitro fertilization of eggs in our hospital from July 2016 to October 2019 were selected. Embryo (3PN) was selected and subdivided into the following four groups according to different fragment contents: low fragment group (fragment proportion <10%), medium and low fragment group (25%> fragment proportion ≥10%), medium and high fragment group (50%> fragment proportion ≥25%), and high fragment group (fragment proportion ≥50%), with 60 cases in each group. The selected embryos were cultured in G2 blastocyst medium and interferon (40ng/mL) interferon was used for 36h. Beclin1, PI3K, LC3B, mTOR and AKT were quantitatively determined by western blot. Results: Before and after interference, Beclin1 protein, PI3K protein, LC3B protein, mTOR protein and AKT protein content in the four groups were significantly increased, with statistically significant differences (P<0.05). Before interference, the contents of the six proteins were compared, from high to low: low fragment group, medium and low fragment group, medium and high fragment group, and high fragment group, with statistically significant differences (P<0.05). After infection, the contents of Beclin1 protein, PI3K protein and LC3B protein in the four groups were significantly different (P<0.05). Beclin1 was significantly correlated with PI3K, LC3B, mTOR and AKT (P<0.05). Conclusion: A high proportion of embryo fragments will inhibit Beclin1, PI3K, LC3B, mTOR and AKT protein content. Beclin1's regulation of autophagy may produce interferon-like effects, thus affecting the expression of related channel proteins.

Key words： Beclin1 Autophagy Embryo fragments Channel protein

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.10.004 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I10/1600

[1] Klohonatz KM,Nulton LC,Hess AM ,et al.The role of embryo contact and focal adhesions during maternal recognition of pregnancy[J].PLoS One,2019,14(3):213322.
[2] Sessions-Bresnahan DR,Heuberger AL,Carnevale EM. Obesity in mares promotes uterine inflammation and alters embryo lipid fingerprints and homeostasis[J].Biol Reprod,2018,99(4):761~772.
[3] 李亚梅,杨星宇,赵雪娅,等.早发型和晚发型子痫前期自噬与凋亡水平差异及相互关系研究[J].中国计划生育和妇产科,2019,11(11):38~43,97.
[4] Donoughe S,Kim C,Extavour CG .High-throughput live-imaging of embryos in microwell arrays using a modular specimen mounting system[J]. Biol Open,2018,7(7):10~12.
[5] Kordus RJ,LaVoie HA .Granulosa cell biomarkers to predict pregnancy in ART: pieces to solve the puzzle[J].Reproduction ,2017 ,153(2):69~83.
[6] 付蒙,苏雪松,吕晓杰,等.授精第3日胚胎的囊胚培养结果及影响因素探讨[J].中国临床医生杂志,2018,46(6):737~739.
[7] 施文浩,张四林,王永博,等.植入前遗传学筛查中不同形态特征的胚胎整倍体率比较[J].中国妇幼健康研究,2018,29(1):45~48.
[8] 梁泳娜,谢斯盛,梁见弟,陈燕锋.精子DNA碎片指数对评估体外受精-胚胎移植患者妊娠结局的临床价值[J].中国优生与遗传杂志,2019,27(1):95~98.
[9] Rusmini P,Cortese K,Crippa V,et al.Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration[J].Autophagy,2019 ,15(4):631~651.
[10] Raimondi M,Cesselli D,Di Loreto C,et al.USP1 (ubiquitin specific peptidase 1) targets ULK1 and regulates its cellular compartmentalization and autophagy[J].Autophagy,2019,15(4):613~630.
[11] Zheng Y,Tan K,Huang H.Long noncoding RNA HAGLROS regulates apoptosis and autophagy in colorectal cancer cells via sponging miR-100 to target ATG5 expression[J].Cell Biochem,2019,120(3):3922~3933.
[12] Fusco C,Mandriani B,Di Rienzo M,et al.TRIM50 regulates Beclin 1 proautophagic activity[J].Biochim Biophys Acta Mol Cell Res,2018,1865(6):908~919.