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| The Role and Mechanism of Vitamin D in Regulating Vascular Endothelial Growth Factor in Neonatal Rats with Broncho Pulmonary Dysplasia |
| ZHAO Ting, WANG Yanmei, WANG Le |
| The First Affiliated Hospital of Xinjiang Medical University, Xinjiang Urumqi 830054, China |
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Abstract Objective: To study the effect of VEGF regulated by vitamin D (VD) and/or VD receptor (VDR) on bronchopulmonary dysplasia (BPD) in neonatal rats. Methods: BDP neonatal rats were constructed and divided neonatal rats into control group and BDP group. Rat lung epithelial type Ⅱ cells (RLE-6TN) were cultured, and divided into VDR overexpression group (Le-VDR lentivirus infection group), VDR overexpression control group (Le-Ctrl group), VDR knockdown group (Sh-VDR infection group), VDR knockdown control group (Sh-Ctrl group), VD treatment alone group, VEGF-A silencing group (siVEGF-A group), and Le-VDR and siVEGF-A co-treatment group (Le-VDR + siVEGF-A group). The regulated effect of VD and VDR on lung cells was confirmed by CCK-8 detection, flow cytometry, wound healing detection and in vitro adhesion detection. VDR and VEGF-A mRNA were detected by RTqPCR, and VDR and VEGF-A protein were detected by Western blot. Results: Compared with the control group, the VD level and VDR expression in BPD neonatal rats were downregulated (P<0.01); overexpression of VDR in RLE-6TN cells increased the cell proliferation, migration, and adhesion ability (all P<0.01), and the apoptosis rate were decreased (P<0.01); however, the effect of knocking down VDR in cells was opposite. VD stimulation inhibited cell apoptosis and increased cell proliferation, migration and adhesion (P<0.05); VDR overexpression positively regulated vascular endothelial growth factor (VEGF)-A (P<0.05); Moreover, VEGF-A and VDR were co-localized in the lung tissue and cells of BPD rats; furthermore, the expression levels of VEGF-A in lung tissue of BPD neonatal rats were decreased (P<0.05). Conclusion: VD inhibits the development of BPD by up-regulating the expression of VEGF-A.
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2020, Vol. 26 
