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| An Analysis of the Effects of MicroRNA-663b on Cell Activity and Apoptosis of Human Intervertebral Disc Degeneration Nucleus Pulposus Cells Induced by Low Glucose |
| WANG Peng, ZUO bin, TANG Jiaguo, et al |
| Changjiang Shipping General Hospital, Hubei Wuhan 430014, China |
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Abstract Objective: To study the regulatory mechanism of miR-663b on the proliferation and apoptosis of human intervertebral disc degeneration nucleus pulposus cells induced by low glucose. Methods: The expression of miR-663b in degenerative nucleus pulposus cells of human disc herniation and glucose (5, 1 mmol/L) -treated discs was detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. The low glucose group + miR-NC group (transfected with miR-NC), the low glucose group + miR-663b group (transfected with miR-663b mimics), and the low glucose group + anti-miR-NC group (transfected with anti-miR -NC), low glucose group + anti-miR-663b group (transfected with anti-miR-663b), divided with the plastid method, were transfected into 1 mmol/L treated disc degeneration nucleus pulposus cells. Cell Counting Kit (CCK-8) method, flow cytometry, and western blot were used to detect the absorbance (OD) value of cells, the apoptosis rate of cells, and cell cycle-dependent protein kinase inhibitor 1A (P21) , protein expression of cysteine aspartic protease (Caspase-3) in cells. Results: The expression of miR-663b in tissues of human disc herniation and 1 mmol/L glucose (low glucose group) treated disc degeneration nucleus pulposus cells were significantly reduced (P<0.05). The cells of the low glucose group were inhibited from proliferating, their apoptotic capacity was improved, and the protein expression of P21 and Caspase-3 was increased. The expression of miR-663b was significantly increased in the low-glucose group cells overexpressing miR-663b, the cell proliferation ability was also significantly increased, and the apoptotic capacity was significantly reduced. The protein expression levels of P21 and Caspase-3 were inhibited, while miR-663b's low-glucose group cells had opposite effect. Conclusion: MiR-663b can promote the proliferation of human intervertebral disc degeneration nucleus pulposus cells induced by low glucose and inhibit apoptosis.
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2020, Vol. 26 
