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| The Effect of Radiotherapy on DC Cell in Peripheral Blood of Patients with Lung Cancer |
| BAI Lu, LIU Chengyi, ZHANG Jingjing, et al |
| The Affiliated hospital of Chengde Mediacal Collage, Hebei Chengde 067000, China |
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Abstract Objective: To discuss the dynamic changes of DC cell in peripherd blood of patients with lung cancer before and after different radiotherapy dose. Methods: 30 patients with lung cancer were included in the study. To detect DC cell count, live DC cell number, DC number of apoptotic cells and DC cell activity in Peripheral blood of 30 lung cancer radiotherapy patients by MACS(magnetic activated cell sorting) before and after radiotherapy. Results: The number of DC cells increased gradually at 2000cGy, 4000cGy and 6000cGy by radiotherapy, F=8.53, P < 0.001. The number of live DC cells increased at first and then decreased. The highest level at 4000cGy by radiotherapy, and declined at 6000cGy, and 4000cGy by radiotherapy, F=6.88 and P < 0.05 compared with 2000cGy and 6000cGy by radiotherapy. There was no significant change in the number of apoptotic cells in DC, which was the highest at 4000cGy by radiotherapy. The dose of 6000cGy compared with 1 week before radiothrapy, the dose of 2000cGy and 4000cGy, F=3.68 and P=0.015. The changes of the activity of DC cells in the serum of the patients with lung cancer before and after radiotherapy have no significant difference in the activity of DC cells, F=0.839, P=0.476. Conclusion: Radiation therapy can increase the level of DC cells in peripheral blood of lung cancer patients and promote the action of tumor antigen.
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2020, Vol. 26 
