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| A Study on the Correlation Between CDKN2B-AS1 Gene Polymorphism and Endometriosis |
| HAO Na, et al |
| Department of Gynecology, Handan Central Hospital, Hebei Handan 056001, China |
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Abstract Objective: To study the correlation between the gene polymorphism of cyclin dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) and endometriosis (EM). Methods: From December 2017 to July 2019, 112 patients with endometriosis admitted to Handan Central Hospital and the Fourth Hospital of Hebei Medical University were selected as the case group, and another 112 patients who were operated on due to ectopic pregnancy and tubal adhesion were selected as the control group. Detection of CDKN2B-AS1 gene polymorphism in blood of all subjects was made by DNA PCR sequencing, the relationship between rs10965235 polymorphism of CDKN2B-AS1 gene and clinical stage of EM was analyzed, and Logistic regression was used to analyze the effect of CDKN2B-AS1 gene polymorphism on EM susceptibility. Results: There was no significant difference in age, BMI, menstrual cycle, frequencies of allele A, G, AA, AG+GG at rs4977574 loci on CDKN2B-AS1 between the case group and the control group (P>0.05). Compared with the control group, the frequencies of alleles C and CC at rs10965235 on CDKN2B-AS1 gene in the case group were higher, and the frequencies of alleles A and CA+AA were lower (χ2=4.397, 4.075, P=0.036, 0.044) . Compared with AA genotype, GA + GG genotype at rs4977574 on CDKN2B-AS1 gene was not related to EM susceptibility (OR=1.105, 95% CI: 0.819~1.491; P=0.124). Compared with I ~ II phase, the frequency of CC genotype at rs10965235 on CDKN2B-AS1 gene in III ~ IV phase EM patients was higher, and the frequency of CA + AA genotype at rs10965235 loci on CDKN2B-AS1 gene was lower, and the differences were statistically significant (χ2=5.168, P=0.023). Compared with CC genotype, CA + AA genotype at rs10965235 on CDKN2B-AS1 gene could reduce EM susceptibility (OR=0.564, 95% CI: 0.347~0.917, P=0.000). Conclusions: The rs4977574 loci on CDKN2B-AS1 gene is not related to EM susceptibility, and the rs10965235 loci polymorphism is related to EM susceptibility of women in northern China.
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2020, Vol. 26 
