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| Effects of miR-655-3p on the Cellular Proliferation and Invasion of Head and Neck Squamous Cell Carcinoma Through ZEB1 Targeted Inhibition |
| ZHAO Lei, XIAO Erbin, LIANG Jingwei, et al |
| The Affiliated Hospital of Hebei University, Hebei Baoding 071000, China |
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Abstract Objective: To study the expression of miR-655-3p in head and neck squamous cell carcinoma (HNSCC) tissues and cell lines and its effects on the biological functions of HNSCC cell lines. Methods: The expression levels of miR-655-3p in HNSCC tissues and cell lines (FaDu, SCC-9, TU686, TU212) were measured by qRT-PCR, and the effects of miR-655-3p on cellular proliferation, migration and invasion were evaluated by a series of assays. The possible target gene of miR-655-3p was further screened and its mechanism was explored. Results: The expression level of miR-655-3p in HNSCC was lower than that in control group, P<0.05. And the over-expression of miR-655-3p in HNSCC cell lines significantly inhibited the proliferation, migration and invasion of cell lines. Further mechanism studies showed that ZEB1, as the target gene of miR-655-3p, was negatively regulated by miR-655-3p. Even, miR-655-3p inhibited the epithelial mesenchymal transformation of HNSCC cell lines by targeting ZEB1 expression directly. Conclusion: MiR-655-3p is down-regulated in HNSCC cancer tissues and cell lines, which could target the expression of ZEB1 directly and inhibit the progression of malignant phenotype of cell lines.
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[1] Nowicka Z, Stawiski K, Tomasik B, et al. Extracellular miRNAs as biomarkers of head and neck cancer progression and metastasis [J]. Int Mol Sci, 2019, 20(19):4799.
[2] Subramaniam S, Jeet V, Clements JA,et al. Emergence of microRNAs as key players in cancer cell metabolism [J]. Clin Chem, 2019, 65(9):1090~1101.
[3] Khan AQ, Ahmed EI, Elareer NR, et al. Role of miRNA-regulated cancer stem cells in the pathogenesis of human malignancies [J]. Cells, 2019, 8(8):E840.
[4] Valenti F, Sacconi A, Ganci F, et al. The miR-205-5p/BRCA1/RAD17 axis promotes genomic instability in head and neck squamous cell carcinomas [J]. Cancers (Basel), 2019, 11(9):1347.
[5] Zhang M, Li Q, Pan Y, et al. MicroRNA-655 attenuates the malignant biological behaviours of retinoblastoma cells by directly targeting PAX6 and suppressing the ERK and p38 MAPK signalling pathways[J]. Oncol Rep, 2018, 39(4):2040~2050.
[6] Kiuchi J, Komatsu S, Imamura T, et al. Low levels of tumour suppressor miR-655 in plasma contribute to lymphatic progression and poor outcomes in oesophageal squamous cell carcinoma [J]. Mol Cancer, 2019, 18(1):2.
[7] Wang W, Cao R, Su W, et al. miR-655-3p inhibits cell migration and invasion by targeting pituitary tumor-transforming 1 in non-small cell lung cancer [J]. Biosci Biotechnol Biochem, 2019, 83(9):1703~1708.
[8] Krebs AM, Mitschke J, Lasierra Losada M, et al. The EMT-activator Zeb1 is a key factor for cell plasticity and promotes metastasis in pancreatic cancer [J]. Nat Cell Biol, 2017, 19(5):518~529.
[9] FERLAY J, SOERJOMATARAM I, DIKSHIT R, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012 [J]. Int Cancer, 2015, 136(5): E359~386.
[10] Zhao Z, Gao D, Ma T, et al. MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma [J]. Aging (Albany NY), 2019, 11(3):921~932.
[11] Zhao Z, Yang S, Cheng Y, et al. MicroRNA-655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular endothelial growth factor [J]. Mol Med Rep, 2018, 18(2):1878~1884. |
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2020, Vol. 26 
