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| Paeonol Alleviates Myocardial Ischemia Reperfusion Injury in Rats by Promoting SIRT1 |
| LIU Chao, et al |
| Affiliated Hospital of Chengde Medical University, Hebei Chengde 067000, China |
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Abstract Objective: To detect the protective effects of paeonol on myocardial ischemia reperfusion (IR) in rats and explore the underlying mechanism. Methods: Sprague Dawley (SD) rats were used to establish myocardial ischemia-reperfusion injury model. Western blot was used to determine the expression level of sirtuin1 (SIRT1). Reactive oxygen species (ROS) of rats' myocardium tissue was analyzed by DCFDA. Oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) and myocardial injury markers lactate dehydrogenase (LDH) and creatine kinase (CK) were evaluated by ELISA. Echocardiography was used for determining myocardial functions and left ventricular remodeling. Results: ROS generation was significantly decreased by paeonol pre-treatment. Besides, after paeonol treatment, MDA, LDH and CK were notably inhibited and SOD was elevated. Myocardial functions and left ventricular remodeling were improved by paeonol. Furthermore, paeonol significantly promoted SIRT1 and the effects were reversed by knocking down SIRT1. Conclusion: Paeonol alleviates oxidative stress and myocardial injury led by ischemia reperfusion, and thus improves myocardial functions and ventricular remodeling by promoting SIRT1.
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2020, Vol. 26 
