基质金属蛋白酶-9基因多态性与2型糖尿病视网膜病变的相关性研究

doi:10.3969/j.issn.1006-6233.2017.10.023

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Abstract Objective: To study of the association between polymorphism of the MMP-9 gene(-1562C/T)and type 2 diabetic retinopathy. Methods: MMP-9 -1562C/T SNP was genotyped by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) ,analysis in 180 healthy persons as control and 400 patients with type 2 diabetes including 180 complication-free cases and 220 with diabetic retinopathy (DR). Results: Association between the MMP-9 -1562C/T SNP in promoter region and diabetic retinopathy: the C and T allele frequencies in Control, DM, DR groups are 8.3%,91.7%; 7.8%, 92.2%; 91.4%, 8.6% respectively. And the results have a significant deviation (χ²=790.09,P=0.000). The C/C, C/T , T/T genotypic frequencies of cases in the three groups are 0%,16.7%,83.3%;2.2%,11.1%,86.7%;84.1%,14.5%,1.4%.There's a significant deviation of C and T allele frequency of MMP-9 -1562C/T between the Control group、the DM group and DR group(χ²=470.16,P=0.000).There's a significant deviation of C/C,C/T,T/T genotypic frequencies between the Control group and DM group(P<0.01),or the DM group and DR group(P<0.01),while there is no significant deviation between the Control group and DM group(P>0.01) .Compared with the T allele, the C allele may increase the onset risk of diabetic retinopathy. Conclusion: The MMP-9 -1562C/T SNP may play a role in the genetic susceptibility to the invasion of diabetic retinopathy. T allele is a protective gene for diabetic retinopathy.

Key words： Diabetic retinopathy Matrix metalloproteinase-9 Gene Single nucleotide polymorphism

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2017.10.023 OR http://www.hbyxzzs.cn/EN/Y2017/V23/I10/1663

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