|
|
|
| Effects of Momordica Saponin on Proliferation Apoptosis and JAK / STAT5 Pathway of Human Malignant Melanoma Skmel-2 Cells |
| HUANG Cuimin, YE Xiuqun, LUO Yueji |
| The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Guangzhou 510405, China |
|
|
|
|
Abstract Objective: To investigate the effects of balsam pear saponins on the growth of human malignant melanoma SKMEL-2 cells and JAK/STAT5 pathway. Methods: Cultured human malignant melanoma SKMEL-2 cells were treated with balsam pear saponins at concentrations of 1, 2.5, 5, 7.5 and 10 mg/mL. The proliferation of SKMEL-2 cells was detected by CCK-8 method after 24, 48 and 72 hours, respectively, and the appropriate concentration and time of drug action were screened. SKMEL-2 cells cultured in vitro were randomly divided into three groups: control group, balsam pear saponin group and cisplatin group. After drug treatment, CCK-8 was used to detect cell proliferation, cell viability was measured, and cell apoptosis was detected by flow cytometry, the apoptotic rate was measured. The expression levels of caspase 3, Bcl-2, Bax, JAK, p-JAK, STAT5 and p-STAT5 were detected by immunoblotting (WB). Results: The inhibitory effect of the drug was higher at 48 hours and 72 hours than that at 24 hours, while the inhibitory effect was basically the same at 48 hours and 72 hours. The proliferation of SKMEL-2 was inhibited by balsam pear saponins at concentrations of 1-5, 7.5 and 10 mg/mL, and the inhibition increased with the increase of concentration, but after 48 and 72 hours, the inhibitory effect tended to be stable when the concentration reached 5 mg/mL. SKMEL-2 cells were treated with balsam pear saponins at a concentration of 5 mg/mL for 48 hours. Compared with control group, the relative survival rate, expressions of p-JAK, p-STAT5 and Bcl-2 in balsam pear saponin group and cisplatin group decreased significantly (P<0.05), apoptotic rate, caspase 3 and Bax expression were significantly increased (P<0.05), there was no significant difference in JAK and STAT5 expressions (P>0.05). There was no significant change in each index between balsam pear saponin group and cisplatin group (P>0.05). Conclusion: Momordica charantia saponins can inhibit the growth of SKMEL-2 cells, promote their apoptosis. It may be the mechanism that momordica charantia saponins can down-regulate the activity of JAK/STAT5 pathway, up-regulate the expressions of caspase 3 and Bax, and inhibit the expression of Bcl-2.
|
|
|
|
|
|
[1] Kunte C, Letule V, Gehl J, et al. Electrochemotherapy in the treatment of metastatic malignant melanoma: a prospective cohort study by insp ECT[J]. Br Dermatol, 2017, 176(6):1475~1485.
[2] Zhu D J, Yin X G, Liu Z Y, et al. Effect of total saponins from bitter gourd to myocardial apoptosis in type 2 diabetic rats[J]. Med West China, 2016, 28(10):1360~1364.
[3] 谢鑫杰,陆熠.苦瓜皂苷G对人肺癌A549细胞的增殖迁移与凋亡的影响及作用机制[J].广西医学,2018,40(9):1068~1072.
[4] Minari J B, Okelola C A, Ugochukwu N C. Analysis of Kras gene from induced pancreatic cancer rats administered with momordica charantia and ocimum ba s ilicum leaf extracts[J].Tradit Complement Med, 2017, 8(2):282~288.
[5] Zhou Y, Leng X, Li H, et al. Tolerogenic dendritic cells induced by BD750 ameliorate proinflammatory T cell responses and experimental autoimmune encephalitis in mice[J]. Mol Med, 2017, 23(1):204~214.
[6] Lobas A A, Pyatnitskiy M A, Chernobrovkin A L, et al. Proteogenomics of malignant melanoma cell lines: the effect of stringency of exome data filtering on variant peptide identification in shotgun proteomics[J].Proteome Res, 2018, 17(5):1801~1811.
[7] 李慧华,徐希明.苦瓜籽活性成分的药理研究进展[J].中医药信息,2019,36(1):125~129.
[8] He X, Sun J, Huang X. Expression of caspase-3, Bax and Bcl-2 in hippocampus of rats with diabetes and subarachnoid hemorrhage[J]. Exp Ther Med, 2018, 15(1):873~877.
[9] Chen L, Lu Z, Yang Y, et al. Effects of purified Omphalia lapidescens protein on metastasis, cell cycle, apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells[J]. Oncol Lett, 2018, 15(4):4161~4170. |
|
|
|
2020, Vol. 26 
