|
|
|
| Research on Biological Role of LncRNA AK093407 in Colorectal Cancer |
| SUN Ao, ZHAO Xuerong, WANG Jianping, et al |
| Chengde Medical Colleg, Hebei Chengde 067000, China |
|
|
|
|
Abstract Objective: To study the biological role of LncRNA AK093407 in human colorectal cancer cells. Methods: QRT PCR was used to detect the expression of lncRNA AK093407 in human colorectal cancer and HCT-116 cells; siRNA silenced the expression of lncRNA AK093407 in colorectal cancer cells: MTT assay was used to detect cell proliferation; annexin V / PI staining was used to detect cell apoptosis; PI staining was used to detect cell cycle; Western staining was used to detect cell cycle. The expression of Caspase-3, caspase-9, caspase-10, Bax, Bcl-2, p21, p27 and p-STAT3 were detected by blot. Results: The expression of lncRNA AK093407 in human colorectal cancer was higher than that in adjacent tissues; the expression of lncRNA AK093407 in HCT-116 was higher than that in NM460; lncRNA was silenced After AK093407, the proliferation activity of HCT-116 cells decreased, apoptosis increased, cell cycle blocked in G1 / S phase; the expression of caspase 3, caspase 9, caspase 10, Bax, Bcl-2, p21, p27 and p-STAT3 decreased. Conclusion: LncRNA AK093407 is highly expressed in colorectal cancer and HCT-116 cells. Silencing lncRNA AK093407 can inhibit cell proliferation, induce cell apoptosis and cell cycle arrest.
|
|
|
|
|
|
[1] Torre L A, Bray F, Siegel R L, et al.Global cancer statistics[J].CA Cancer Clin, 2015, 65(2):87~108.
[2] Bartoniek N, Maag J L, Dinger M E.Long noncoding RNAs in cancer mechanisms of action and technological advancements[J].Mol Cancer, 2016, 15(1):43.
[3] Dong X, Wang J, Li T, Xu Y P, Li S Y.Down regulation of LncRNA MEG3 promotes colorectal adenocarcinoma cell proliferation and inhibits the apoptosis by up-regulating TGF-β1 and its downstream sphingosine kinase1[J].European review for medical and pharmacological sciences, 2018, 23(22):8265~8272.
[4] Zhang L R, Li Jibin, Yan Xiaofei, Jin Keer, Li Wenya, Liu Xin, Zhao Jianfeng et al.Long non-coding RNA MLK7-AS1 promotes proliferation in human colorectal cancer via downregulation of p21 expression[J].Molecular, 2018, 19(2):1210~1221.
[5] Yongkun Wang, Tingting Liang, Yao Wang, Yan Huang, Ye Li.Long non-coding RNA AK093407 promotes proliferation and inhibits apoptosis of human osteosarcoma cells via STAT3 activation[J].Am Cancer Res, 2017, 7(4):892~902.
[6] Liu Xiaoli, Dong Jinhu, Cai Wangyu, Pan Ying, Li Ruili, Li Bing.The effect of thymoquinone on apoptosis of SK-OV-3 ovarian cancer cell by regulation of Bcl-2 and Bax.[J].International Journal of Gynecological Cancer, 2017, 8(27):1596~1601.
[7] Xiaopeng Yu, Bo Huang, Zhi Zhou, Jia Tang, Yang Yu.Involvement of caspase3 in the acute stress response to high temperature and elevated ammonium in stony coral Pocillopora damicornis[J].Gene, 2017, 637:108~114.
[8] Kiraz Y, Adan A, Kartal Yandim M and Baran Y: Major apoptotic mechanisms and genes involved in apoptosis[J]. Tumour Biol,2016,37: 8471~8486.
[9] Tan HY, Wang C, Liu G, Zhou X.Long noncoding RNA NEAT1-modualted miR-506 regulates gastric cancer development through targeting STAT3[J].Cell Biochem, 2018, 120(4):4827~4836.
[10] Chen H, Pan J, Zhang L, Chen L, Qi H, Zhong M, Shi X, Du J, Li Q.Downregulation of estrogen-related receptor alpha inhibits human cutaneous squamous cell carcinoma cell proliferation and migration by regulating EMT via fibronectin and STAT3 signaling pathways[J].Eur Pharmacol, 2018, 825:133~142.
[11] Chen JF, Wu P, Xia R, Yang J, Huo XY, Gu DY, Tang CJ, De W, Yang F.STAT3-induced LncRNA HAGLROS overexpression contributes to the malignant progression of gastric cancer cells via mTOR signal-mediated inhibition of autophagy[J].Mol Cancer, 2018, 17(1):6. |
|
|
|
2020, Vol. 26 
