胡桃醌通过调节CDK4表达调控口腔鳞癌Tca8113细胞增殖和凋亡

doi:10.3969/j.issn.1006-6233.2020.02.038

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Abstract Objective: To investigate effect of juglone on proliferation and apoptosis of oral squamous cell carcinoma Tca8113 cells by regulating CDK4 expression. Methods: Oral squamous cell carcinoma Tca8113 cells were divided into 4 groups: control group, low-dose, middle-dose, high-dose juglone groups (0μmoL/L, 25μmoL/L, 50μmoL/L, 100μmoL/L). The morphological changes of cells in different concentration groups were observed by inverted microscope.The inhibitory effect of juglone on the proliferation of Tca8113 cells was determined by MTT assay. The effect of walnut juglone on Tca8113 cell cycle distribution was analyzed by flow cytometry. The expression of CDK4 in Tca8113 cells was analyzed by immunocytochemistry. Results: 24 hours after the action of walnut quinone, the number of cells was decreased, the cytoplasm became rough, the cells became smaller and rounder, and the higher the concentration of walnut quinone, the more obvious the change was. MTT results showed that OD values of Tca8113 cells in low, medium and high dose groups were lower than those in control group (P<0.05), OD values of Tca8113 cells in medium and high dose groups were lower than those in low dose group (P<0.05), apoptotic rates in high, medium and low dose groups were significantly higher than those in control group (P<0.05), and apoptotic rates in high, medium and low dose groups were higher than those in low dose group (P<0.05). The positive rate of CDK4 in high-dose group was higher than that in medium-dose group (P<0.05); the positive rate of CDK4 in high, middle and low-dose group was lower than that in control group (P<0.05); the positive rate of CDK4 in high and middle-dose group was lower than that in low-dose group (P<0.05); and the positive rate of CDK4 in high-dose group was lower than that in middle-dose group (P<0.05). Conclusion: Juglone can inhibit the proliferation of oral squamous cell carcinoma Tca8113 cells, induce apoptosis, down-regulate the expression of CDK4 protein, and inhibit the invasion of tumors.

Key words： Juglone CDK4 Oral squamous cell carcinoma Tca8113

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.02.038 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I2/329

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