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| Role of Connective Tissue Growth Factor PD98059 and LY294002 in Regulating the Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells of Rat |
| HU Yu, ZHOU Mengjun, et al |
| MianYang Central Hospital, Sichuan Mianyang 621000, China |
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Abstract Objective: To explored the proliferation and migration of (PASMC) in rat pulmonary artery smooth muscle cells under the intervention of connective tissue growth factor (CTGF), PD98059 and LY294002. Method: PASMC was isolated and cultured, and then were divided into blank group, CTGF group, CP Group, CL group and CPL group. The effects of CTGF, PD98059 and LY294002 on cell proliferation were detected by WST-1, and the effects on cell migration were detected by Transwell, cell scratch and cytoskeleton. Results: Compared with the blank group, the proliferation level of WST-1 cells increased after CTGF (50NG/ml) stimulation of pasmc48, 72, 96 and 120 hours, the number of transmembrane cells increased after 6h and 12h, the scratch width of cells decreased after 12h and 24h, and the cytoskeleton was fuzzy, the difference was statistically significant (P<0.05). After PD98059 (20 μ mol / L) and / or LY294002 (10 μ moL/L) intervention for 24, 48, 72, 96 and 120 hours, WST-1 showed a decrease in cell proliferation; after 6 and 12 hours, Transwell showed a decrease in the number of transmembrane cells, and after 12 and 24 hours, the scratch width and cytoskeleton of the cells were increased, the difference was statistically significant (P<0.05). Conclusion: CTGF can stimulate the proliferation and migration of PASMC and may be one of the factors to promote the pulmonary vascular remodeling of pulmonary hypertension (PAH). PASMC proliferation and migration can be inhibited by PD98059 and / or LY294002, suggesting that ERK1 / 2 and PI3K pathways blocked by CTGF may participate in the pulmonary vascular remodeling of PAH stimulated by CTGF.
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[1] Ming Lu,Ying Luo,Pengfei Hu,et al.Tanshinone IIA inhibits AGEs-induced proliferation and migration of cultured vascular smooth muscle cells by suppressing ERK1/2 MAPK signaling[J].Iran Basic Med Sci,2018,21(1):83~88.
[2] Jasmine Walker,Clark Undem,Xin Yun,et al. Role of Rho kinase and Na+/H+ exchange in hypoxia-induced pulmonary arterial smooth muscle cell proliferation and migration[J].Physiol Rep,2016,4(6): e12702.
[3] Justin R. Sysol,Viswanathan Natarajan,Roberto F. Machado.PDGF induces SphK1 expression via Egr-1 to promote pulmonary artery smooth muscle cell proliferation[J].Am Physiol Cell Physiol,2016,310(11): C983~C992.
[4] Bradley A. Maron, William M. Oldham, Stephen Y. Chan,et al. Upregulation of steroidogenic acute regulatory protein by hypoxia stimulates aldosterone synthesis in pulmonary artery endothelial cells to promote pulmonary vascular fibrosis[J]. Circulation,2014,130(2): 168~179.
[5] Nikolaos G. Frangogiannis.The role of transforming growth factor (TGF)-β in the infarcted myocardium[J].Thorac Dis,2017,9(Suppl 1): S52~S63.
[6] Li Chen,David R Brigstock.Integrins and heparan sulfate proteoglycans on hepatic stellate cells (HSC) are novel receptors for HSC-derived exosomes[J]. FEBS Lett,2016,590(23): 4263~4274.
[7] Yoolhee Yang,Hee Jung Kim,Kyong-Je Woo, et al.Lipo-PGE1 suppresses collagen production in human dermal fibroblasts via the ERK/Ets-1 signaling pathway[J]. PLoS One,2017,12(6): e0179614.
[8] Lun Dong,Fanyan Meng,Ling Wu,et al.Cooperative oncogenic effect and cell signaling crosstalk of co-occurring HER2 and mutant PIK3CA in mammary epithelial cells[J]. Int Oncol,2017,51(4): 1320~1330.
[9] Kai Yang,Kai Gao,Gui Hu, et al.CTGF enhances resistance to 5-FU-mediating cell apoptosis through FAK/MEK/ERK signal pathway in colorectal cancer[J]. Onco Targets Ther,2016,9: 7285~7295. |
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2020, Vol. 26 
