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| Effect of miR-663 on the Growth Proliferation Migration and Invasion of PG-LH7 and PG-BE1 Cells |
| WANG Yunpeng, et al |
| North China University of Science and Technology, Hebei Tangshan 063000, China |
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Abstract Objective: To explore the modulating effect of the microRNA-663 (miR-663) on the proliferation ability and invasive ability in human lung cancer cells PG-LH7 and PG-BE1. Methods: Real time quantitative RT-PCR was used to detect the expression of miR-663 in A549 cells, PG-LH7 cells, PG-BE1 cells, PG-BE1 cells transfected by mir-663ASO and PG-LH7 cells transfected by miR-663mimics; cell count, colony forming assay and CCK8 method were used to detect the proliferation of PG-BE1 cells and PG-LH7 cells transfected by miR-663ASO or miR-663mimics; Transwell cell was used to detect the expression of miR-663ASO or miR-663mimics after transfection The invasion and migration ability of PG-BE1 cells and PG-LH7 cells. Results: The expression level of miR-663 in PG-LH7 cells was lower than that in A549 cells (P<0.05), the expression level of miR-663 in PG-BE1 cells was higher than that in A549 cells (P<0.05), the expression level of miR-663 in PG-LH7 was increased by miR-663mimics transfection (P<0.05), the expression level of miR-663 in PG-BE1 was significantly decreased by miR-663ASO transfection (P<0.05), and the expression level of miR-663mimics was effective MiR-663ASO can effectively inhibit the proliferation and invasion of PG-BE1 cells (P<0.05). Conclusion: Overexpression of miR-663 can promote the proliferation, invasion and migration of PG-LH7, a low metastasis sub line of human lung cancer, while inhibition of miR-663 can inhibit the growth, proliferation, migration and invasion of PG-BE1, a high metastasis sub line.
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2020, Vol. 26 
