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| MiR-23a Promoting Cell Proliferation of Human Tongue Squamous Cell Carcinoma Cell through Regulating PPP2R5E |
| TAO Yadong, et al |
| Affiliated Hospital of Chengde Medical University, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the effect mechanism of miR-23a on the proliferation of human tongue squamous cell carcinoma cells. Methods: Clinical tissue samples of tongue squamous cell carcinoma were collected and Tca8113 and CAL27 were cultured. Real-time quantitative PCR was performed to detect the expressions of miR-23a and PPP2R5E in Clinical tissue samples of tongue squamous cell carcinoma. MTT assay, colony formation assay and growth curve assay were used to detect the effect of miR-23a and PPP2R5E on the proliferation of human tongue squamous carcinoma cell. Luciferase reporter assay verified the regulatory relationship between mir-23a and PPP2R5E. Results: The expression of miR-23a in tongue squamous cell carcinoma was significantly up-regulated(P<0.01). MiR-23a promoted the proliferation of tongue squamous cell carcinoma cells. Bioinformatics prediction and luciferin reporting experiments showed that PPP2R5E was a direct target gene of miR-23a. The expression of PPP2R5E was decreased in tongue squamous cell carcinoma. PPP2R5E inhibited the proliferation of tongue squamous cell carcinoma cells. Overexpression of PPP2R5E can reverse the proliferation promoting effect of mir-23a on tongue squamous cell carcinoma cells. Conclusion: MiR-23a can promote the proliferation of tongue squamous cell carcinoma cells through PPP2R5E. MiR-23a plays an oncogene role in the occurrence and development of tongue squamous cell carcinoma.
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2020, Vol. 26 
