Lnc RNA PCGEM1通过TGF β2/Smad2信号通路调控食管癌细胞侵袭和转移研究

doi:10.3969/j.issn.1006-6233.2020.01.014

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Abstract Objective: To investigate the regulation of long-chain noncoding RNA PCGEM1(Lnc RNA PCGEM1)in the invasion and metastasis of esophageal cancer cells through the TGF β2/Smad2 signaling pathway. Methods: Cancer tissues and adjacent normal tissues of 62 EC patients were collected. QRT-pPCR was used to detect the expression of Lnc RNA PCGEM1 in EC and corresponding paracancer tissues and different cells. Lnc RNA PCGEM1 silenced cell lines were constructed, and the cells were divided into Eca-109-siPCGEM1 group, negative control Eca-109-siNC group, and Eca-109 as blank control group.MiR-148a silenced cell lines of Eca-109-siPCGEM1 cells were constructed, which were divided into siPCGEM1+ simiR-148a group and siPCGEM1+siNC group.Eca-109 cell proliferation was detected by plate cloning assay.The effect of Eca-109 cell migration was detected by scratch assay. The bioinformatics website StarBase was used to predict the complementary binding microRNA (miRNA) of PCGEM1, and the targeted binding genes of corresponding miRNA were predicted based on the Targetscan website. Western Blot analysis of TGF β2/Smad2 signaling pathway protein expression. Results: QRT-PCR results showed that the expression level of Lnc RNA PCGEM1 in EC tissues and esophageal cancer cell lines was higher than that in adjacent normal tissues (P<0.05). Compared with other cell lines, Lnc RNA PCGEM1 had the highest expression in Eca-109 cells (P<0.05). Compared with the blank control group, the number of cell clones and migration in the Eca-109-siPCGEM1 group was significantly reduced, and the expression of miR-148a was significantly increased (P<0.05)..There was no significant difference between the blank control group and Eca-109-siNC group (P>0.05).Compared with the siPCGEM1+siNC group, the number of cell clones, number of migration were significantly increased in the siPCGEM1+ simiR-148a group (P<0.05). The prediction results of StarBase website showed that Lnc RNA PCGEM1 could be complementary to miR-148a, and the prediction analysis of Targetscan website showed that miR-148a had a targeted binding site with TGF β2.Western blot results showed that the expressions of TGF β2 and Smad 2 in Eca-109-siPCGEM1 group were significantly lower than those in blank control group and Eca-109-siNC group (P<0.05).The expression levels of the above indicators in the blank control group and Eca-109-siNC group were not statistically significant (P>0.05). Conclusion: Lnc RNA PCGEM1 is highly expressed in esophageal cancer. High expression of Lnc RNA PCGEM1 may enhance the TGF β2/Smad2 signaling pathway by downregulation of miR-148a, thus promoting the development of EC.

Key words： Esophageal cancer Lnc RNA PCGEM1 MiR-148-a TGF β/Smad signaling pathway

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	LIU Yong
	WANG Bin
	JIN Jianqin
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LIU Yong,WANG Bin,JIN Jianqin, et al. Study on Lnc RNA PCGEM1 Regulating the Invasion and Metastasis of Esophageal Cancer Cells through the TGF beta/Smad Signaling Pathway[J]. HeBei Med, 2020, 26(1): 58-62.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.01.014 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I1/58

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