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| Screening Maternal Serum Protein Markers of Down's Syndrome Based on Mass Spectrometry |
| XIAO Yanping, FU Jiuyuan, GE Yongmei, et al |
| The Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China |
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Abstract Objective: To detect specific maternal serum protein markers for Down's syndrome early. Methods: Ten maternal sera (14~20 weeks) and 10 normal maternal sera at the same time point were collected. The differential expression proteins were analyzed by mass spectrometry and further verified by Western blot. Results: By mass spectrometry analysis, 13 proteins with abnormal expression of more than 1.0 times were found in maternal serum of Down syndrome fetuses. DAVID functional analysis showed that 13 differentially expressed proteins were mainly involved in lipoprotein biosynthesis and decomposition, apoptosis and platelet degranulation. The abnormal expression of FN1 and IGFBP3 in maternal serum of Down's syndrome fetus was 2.5 times higher than that in normal control group (P<0.05). Conclusion: Mass spectrometry is an effective method for screening maternal serum protein markers of Down syndrome, and FN1 and IGFBP3 may be potential new markers for screening Down syndrome fetuses.
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2019, Vol. 25 
