慢性萎缩性胃炎患者miR-181a和CDX2表达变化及与预后关系

doi:10.3969/j.issn.1006-6233.2019.11.006

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Abstract Objective: To analyze the expressions of micro RNA-181a (miR-181a) and caudal type homologous transcription factor-2 (CDX2) in gastric mucosa, and to explore their relationships with clinicopathological parameters and prognosis in patients with chronic atrophic gastritis (CAG). Methods: 90 patients with CAG admitted from February 2017 to September 2018 were selected as the study subjects, atrophic mucosal biopsy tissues and surrounding normal mucosal biopsy tissues were retained during gastroscopy. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of miR-181a in biopsy tissues, immunohistochemical staining (SP method) was used to detect the expression of CDX2 protein; analyzed their expressions in different tissues and their relationships with clinicopathological parameters and prognosis of CAG patients. Results: The expression level of miR-181a in CAG gastric mucosa was significantly higher than that in normal gastric mucosa (P<0.05), and the positive rate of CDX2 protein was significantly higher than that in normal gastric mucosa (P<0.05). The expressions of miR-181a and CDX2 were not related to sex, Hp infection and chronic inflammation (P>0.05), but to atrophy of gastric mucosa, intestinal metaplasia and atypical hyperplasia in CAG patients (P<0.05). The expressions of miR-181a and CDX2 were related to the prognosis of CAG patients (P<0.05). Conclusion: MiR-181a and CDX2 are highly expressed in gastric mucosa of CAG, their expressions are related to the clinicopathological parameters such as atrophy of gastric mucosa and intestinal metaplasia, and prognosis of CAG patients, which can provide some help for clinical treatment.

Key words： Chronic atrophic gastritis Gastric mucosa tissue Micro RNA-181a Caudal type homologous transcription factor-2

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2019.11.006 OR http://www.hbyxzzs.cn/EN/Y2019/V25/I11/1782

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