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| Effects of Matrine Pretreatment on Nerve cell Apoptosis and Expression of Bax and Bcl-2 Protein in rats with Cerebral Ischemia and Reperfusion |
| ZHAO Yanwu, CAI Chunhua, JIANG Song, et al |
| The Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China |
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Abstract Objective: To observe the effects of matrine pretreatment on the apoptosis of nerve cells and the expression of Bcl-2 and Bax after cerebral ischemia and reperfusion in the brain. Methods: 60 healthy SD rats were randomly divided into 6 groups, namely sham group, model group, matrine group (4, 8, 16 mg/kg), edaravone group (1 mg/kg). The cerebral ischemia reperfusion injury model was established by suture method in rats. Then neuro-behavior was scored in different time, brain cell apoptosis was determined and the expression of Bax and Bcl-2 were examined. Results: Compared by the model group, rats in matrine pretreatment group showed better neurobehavioral score (P<0.05), lower brain cell apoptosis rate (P<0.05), significantly higher expression of bcl-2 protein (P<0.01) and lower expression of Bax protein (P<0.05). Conclusion: Matrine shows a neuro-protective role on cerebral ischemia and reperfusion injury in rats and it enhances the expression of Bcl-2 protein and inhibits the expression of Bax protein.
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2018, Vol. 24 
