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| Effects of Shikonin on cell apoptosis of human liver cancer cell line SMMC-7721 |
| ZHANG Shuling, LIANG Lan, ZOU Ye, et al |
| Chengde County Hospital, Hebei Chengde 067000, China |
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Abstract Objective: To study the effect and mechanism of shikonin on apoptosis of SMMC-7721 cells of liver cancer. Methods: Different concentration of shikonin were used to interfere with liver cancer SMMC - 7721 cells, determined by MTT method to observe the cell proliferation inhibition, and using flow cytometry to test the apoptosis rate after 24h, and further using PCR and Western blot to detect the mRNA and protein expression of caspase3 and caspase9. Results: the growth of liver cancer SMMC-7721 cells was inhibited significantly at 24h, 48h and 72h by shikonin (0, 0.5, 1.0, 2.0, 4.0μg/mL), indicating the dual dependence of time and concentration. According to the results of flow cytometry detection, the apoptosis rate of human liver cancer SMMC-7721 cells was significantly higher than that of the control group after 24 h of action of different concentrations of shikonin (5, 10, 20μg/mL), and the apoptosis rate was significantly increased with the increase of shikonin concentration. In addition, the increased concentration of shikonin can promote the expression of mRNA and protein of caspase3 and caspase9 in human liver cancer SMMC-7721 cells. Conclusion: Shikonin can inhibit the apoptosis of SMMC-7721 cells in liver cancer by promoting the mRNA and protein expression of caspase3 and caspase9.
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2018, Vol. 24 
