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| Correlation Analysis of the Expression Levels of Transmembrane Protein Klotho and Fibronectin in Keloids and Their Relationship with Postoperative Recurrence |
| SUN Yanping, LIU Pengchao, HU Jinhong, et al |
| Chengde Central Hospital, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the correlation between Klotho protein binding to fibronectin Fn and postoperative recurrence in keloids.Methods: A total of 43 patients with ear keloids and 37 general cosmetic seekers who visited our department from April 2020 to April 2023 were selected. The subjects were divided into a study group (keloid tissues and serum) and a control group (normal tissues and serum). Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect and compare the expression of Klotho protein and the epithelial-mesenchymal transition marker fibronectin FN in tissue and serum samples from both groups. Keloid patients were followed up at 1 month, 3 months, 6 months, and 12 months postoperatively to record recurrence, which divided them into recurrence and non-recurrence groups. Statistical methods were used to analyze the correlation between Klotho expression and fibronectin FN expression and postoperative recurrence in keloids.Results: Immunohistochemistry results showed that the positive rate of Klotho in the study group was lower than in the control group (P<0.05), at 37.20%. The positive rate of FN in the study group was 72.10%, higher than in the control group (P<0.05). The positive rate of Klotho in the recurrence group was 21.40%, lower than in the non-recurrence group (P<0.01), and the positive rate of FN in the recurrence group was 92.90%, higher than in the non-recurrence group (P<0.05). There was a statistically significant correlation between the levels of Klotho and FN proteins (r=-0.307, P<0.05). Elisa results showed that the expression level of Klotho in the study group was 65.99 (55.05,79.06) ng/mL, lower than in the control group (P<0.05), and the expression level of FN in the study group was 83.56 (62.67,91.85) ng/mL, higher than in the control group (P<0.01). Logrank tests indicated that negative Klotho expression and positive FN protein expression were associated with postoperative recurrence (both P<0.05).Conclusion: The combination of Klotho protein and the epithelial-mesenchymal transition marker FN can be used as an important marker to predict postoperative recurrence of keloids.
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2024, Vol. 30 
