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| Effect of Oridonin on Apoptosis of Osteoblasts in Osteoporotic Rats by Regulating Hippo/YAP Signaling Pathway |
| LU Haifeng, ZOU Binghong, ZHU Yi |
| Dazhou Orthopedic Hospital, Sichuan Dazhou 635000, China |
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Abstract Objective: To investigate the effect of oridonin (Ori) on osteoblast apoptosis in osteoporosis (OP) rats by regulating the Hippo/Yes-associated protein (YAP) signaling pathway. Methods: Ten rats were randomly selected as the sham surgery group, with only ovaries separated but not removed. The remaining rats were used to establish an OP model by removing both ovaries. The successfully modeled rats were randomly separated into the OP group, different doses of Ori (5 mg/kg Ori, 10 mg/kg Ori, 20 mg/kg Ori) groups, and 20 mg/kg Ori+Hippo/YAP activator - Vesteporfin (10 mg/kg) group. After the intervention, abdominal aortic blood was collected, and ELISA was applied to detect serum levels of osteocalcin (BGP) and alkaline phosphatase (ALP). The femoral tissue was separated, and a dual-energy X-ray bone density instrument was applied to detect femoral mineral content and bone density. HE was applied to detect morphological changes in femoral tissue. TUNEL was applied to detect changes in osteoblast apoptosis. Western blot was applied to detect the expression of YAP and transcription co-activator PDZ binding motif (TAZ) proteins. Results: There was no great change in the femoral structure of the rats in the sham surgery group. Compared with the sham surgery group, there was a great change in the morphology of the femoral tissue in the OP group, the levels of BGP and ALP, femoral tissue mineral content, bone density, YAP, and TAZ protein expression were greatly reduced (P<0.05), the apoptosis rate of osteoblasts was greatly increased (P<0.05). Compared with the OP group, the femoral tissue morphology was improved in the 5 mg/kg Ori group, 10 mg/kg Ori group, and 20 mg/kg Ori group, the levels of BGP and ALP, femoral tissue mineral content, bone density, YAP, and TAZ protein expression were greatly increased (P<0.05), the apoptosis rate of osteoblasts was greatly decreased (P<0.05), there were statistical differences between different doses of Ori (P<0.05). Compared with the 20 mg/kg Ori group, the morphological changes of the femoral tissue in the 20 mg/kg Ori+Verteporfin group were aggravated, the levels of BGP and ALP, femoral tissue mineral content, bone density, YAP, and TAZ protein expression were greatly reduced (P<0.05), the apoptosis rate of osteoblasts was greatly increased (P<0.05). Conclusion: Ori reduces osteoblast apoptosis and alleviates symptoms in OP rats by regulating the Hippo/YAP signaling pathway.
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2024, Vol. 30 
