沉默HMGB1通过抑制p38 MAPK信号通路减少LPS或IL-17A诱导的中耳腔上皮细胞的炎症与凋亡

doi:10.3969/j.issn.1006-6233.2024.05.05

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Abstract Objective: To investigate the regulatory effects and mechanisms of high-mobility group protein B1 (HMGB1) on the inflammatory response and apoptosis of human middle ear epithelial cells (HMEECs) induced by lipopolysaccharide (LPS) or interleukin-17A (IL-17A). Methods: HMEEC cell line was cultured. HMEECs were divided into the control group, LPS group, LPS+shRNA negative control group (LPS+shNC group), LPS+shRNA-mediated HMGB1 knockdown group (LPS+shHMGB1 group), LPS+p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB202190 group (LPS+SB202190 group), IL-17A group, IL-17A+shNC group, IL-17A+shHMGB1 group, and IL-17A+SB202190 group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the changes in the levels of TNF-α, IL-1β and IL-6. Western blot was used to detect the expression of mucin 5AC (MUC5AC), mucin 8 (MUC8), p38 MAPK, phosphorylated p38 MAPK (p-p38 MAPK), E-twenty-six like 1 protein (ELK1), B-cell lymphoma 2 protein (Bcl-2), and Bcl-2 associated X protein (Bax). Flow cytometry was used to detect cell apoptosis. Results: Compared with the control group, the apoptosis rate of HMEECs in the LPS group or IL-17A group was increased, and the expression levels of HMGB1, MUC5AC, MUC8, TNF-α, IL-1β, IL-6, p38, p-p38, ELK1, and Bax were increased, while the expression level of Bcl-2 was decreased (all P<0.05). Compared with the LPS group or IL-17A group, the apoptosis rate was decreased in the LPS+shHMGB1 group or IL-17A+shHMGB1 group, and the expression levels of HMGB1, MUC5AC, MUC8, TNF-α, IL-1β, IL-6, p38, p-p38, ELK1, and Bax were all decreased, while the expression level of Bcl-2 was increased (all P<0.05). Compared with the LPS group or IL-17A group, the apoptosis rate was decreased in the LPS+SB202190 group or IL-17A+SB202190 group, and the expression levels of MUC5AC, MUC8, TNF-α, IL-1β, IL-6, p38, p-p38, ELK1, and Bax were all decreased, while the expression level of Bcl-2 was increased (all P<0.05). Conclusion: Silent HMGB1 alleviates LPS or IL-17A-induced inflammation and apoptosis of HMEECs via inhibiting p38 MAPK signaling pathway.

Key words： High mobility group protein 1 p38 mitogen-activated protein kinase Human middle ear epithelial cells Cell apoptosis

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	ABULAJIANG Tuoheti
	ABULIKEMU Yi
	UUMAIER Yasheng
	et al

Cite this article:

ABULAJIANG Tuoheti,ABULIKEMU Yi,UUMAIER Yasheng, et al. Silent HMGB1 Alleviates LPS or IL-17A Induced Inflammation and Apoptosis of Human Middle Ear Epithelial Cells via Inhibiting p38 MAPK Signaling Pathway[J]. HeBei Med, 2024, 30(5): 724-730.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.05.05 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I5/724

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