miR-1297靶向HMGA1基因以调节胃癌细胞的侵裘和迁移

doi:10.3969/j.issn.1006-6233.2024.05.02

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Abstract Objective: To investigate whether miR-1297 regulates the migration and invasion of gastric cancer by targeting high mobility group A1 (HMGA1). Methods: The expression levels of miR-1297 and HMGA1 in gastric cancer (n=30) and adjacent normal tissues were determined by RT-qPCR. Gastric adenocarcinoma SGC-7901 cell line and human normal gastric mucosal epithelial cells GES-1 were cultured in vitro. SGC-7901 cells were divided into blank group, negative control group, miR-1297 mimics group, miR-1297 inhibitor and miR-1297 inhibitor+HMGA1 siRNA group. Transwell method was used to detect the migration and invasion of SGC-7901 cells. Western blot tests for changes in protein levels. The specific target genes of miR-1297 were detected by luciferase reporter gene assay. Results: The expression of miR-1297 was down-regulated in gastric cancer tissues and SGC-7901 cells, and HMGA1 mRNA level was increased at the same time (P<0.001), and miR-1297 and HMGA1 mRNA expression were negatively correlated (P<0.05). Compared with blank group and negative control group, miR-1297 mimics group could inhibit the migration and invasion ability of cells in vitro (P<0.05). In addition, miR-1297 down-regulates HMGA1 by targeting its 3'-UTR. Compared with blank and negative controls, transfection with miR-1297 inhibitor after downregulation of miR-1297 expression significantly increased cell migration and invasion ability (P<0.05). At the same time, the promoting ability was reversed after transfection of HMGA1 siRNA (miR-1297 inhibitor+HMGA1 siRNA group) (P<0.05). Conclusion: miR-1297 inhibits the migration and invasion of SGC-7901 cells by targeting HMGA1, suggesting that miR-1297/HMGA1 axis provides a new prospective therapeutic strategy for gastric cancer.

Key words： Gastric cancer HMGA1 Invasion Migration miR-1297

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.05.02 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I5/710

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