瑞马唑仑对颅脑损伤大鼠脑组织损伤及TLR4/MyD88/NF-κB通路的影响

doi:10.3969/j.issn.1006-6233.2024.02.01

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Abstract Objective: To investigate the effects of Remimazolam on brain tissue injury and the Toll like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-κB (NF-κB) pathway in rats with traumatic brain injury (BI). Methods: BI rat model was constructed; All rats were grouped into Control group, brain injury group (BI group), low, medium and high dose Remimazolam groups (Rem-L, Rem-M, Rem-H groups), and high dose Remimazolam+TLR4 activator LPS group of (Rem-H+LPS group); the brain tissue water content was detected; ELISA was applied to detect the levels of inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in rat brain tissue; HE staining was applied to observe the morphological changes of brain tissue; TUNEL method was applied to detect neuronal apoptosis in brain tissue; Western blot method was applied to detect the expression of TLR4, MyD88, NF-κB, p-NF-κB p65, Bax, and Bcl-2 proteins in brain tissue. Results: Compared with Control group, brain neurons in BI group were degenerated and necrotic, the number of neurons decreased, the volume decreased, and the injury was serious. Neural function score, brain tissue water content, neuronal apoptosis rate, levels of TNF-α, IL-1β, IL-6 and expressions of Bax, TLR4, MyD88, NF-κB, p-NF-κB p65 were increased. Bcl-2 expression decreased (P<0.05); Compared with BI group, the neuronal cell damage in brain tissue of rats in Rem-L, Rem-M and Rem-H groups was gradually reduced, and the cell structure was relatively clear. The neural function score, brain tissue water content, neuronal apoptosis rate, TNF-α, IL-1β, IL-6 levels and the expressions of Bax, TLR4, MyD88, NF-κB, p-NF-κB p65 were decreased successively, while the expression of Bcl-2 was increased successively (P<0.05); Compared with the Rem-H group, the brain tissue injury of the rats in the Rem-H+LPS group was aggravated, the neural function score, brain tissue water content, neuronal apoptosis rate, the levels of TNF-α, IL-1β, IL-6 and the expressions of Bax, TLR4, MyD88, NF-κB, p-NF-κB p65 were increased, and the expression of Bcl-2 was decreased (P<0.05). Conclusion: Remimazolam can improve brain tissue injury in rats with traumatic brain injury by inhibiting the TLR4/MyD88/NF-κB pathway.

Key words： Remimazolam Traumatic brain injury Toll like receptor 4/myeloid differentiation factor 88/nuclear factor-κB pathway

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.02.01 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I2/177

[1] Graham NS,Sharp DJ.Understanding neurodegeneration after traumatic brain injury:from mechanisms to clinical trials in dementia[J].Neurol Neurosurg Psychiatry,2019,90(11):1221-1233.
[2] 时金昭,刘银龙,桑春生,等.标准去骨瓣减压术在创伤性颅脑损伤治疗中的应用[J].国际外科学杂志,2019,46(7):486-490.
[3] 孙博蕊,孙杰瑜,李志鹏,等.基于TLR4/MyD88/NF-KB通路异甘草素对大鼠颅脑外伤后炎症反应及Th1/Th2失衡的影响[J].西部医学,2022,34(6):785-790.
[4] 姚艳粉,李丕宝,孙玮,等.瑞马唑仑复合右美托咪定镇静对老年创伤性颅脑损伤患者疗效相关指标的影响[J].立体定向和功能性神经外科杂志,2022,35(4):235-240.
[5] Chen X,Pan Z,Fang Z,et al.Omega-3 polyunsaturated fatty acid attenuates traumatic brain injury-induced neuronal apoptosis by inducing autophagy through the upregulation of SIRT1-mediated deacetylation of Beclin-1[J].Neuroinflammation,2018,15(1):310-324.
[6] 郭小丽,杨昌明,王婵,等.基于Sirt1/FoxO1通路探讨瑞马唑仑减轻脓毒症小鼠脑损伤的机制研究[J].中国实验动物学报,2023,31(1):82-90.
[7] 张山,徐伟,韩亚非,等.依达拉奉右莰醇抑制TLR4/NF-κB通路减轻大鼠液压冲击脑损伤[J].脑与神经疾病杂志,2023,31(4):204-209.
[8] 李娜,王瑞辉,郭婕,等.电针对创伤性颅脑损伤大鼠炎症因子表达及神经功能的影响[J].陕西中医,2021,42(10):1358-1362.
[9] Shi M,Chen J,Liu T,et al.Protective Effects of Remimazolam on Cerebral Ischemia/Reperfusion Injury in Rats by Inhibiting of NLRP3 Inflammasome-Dependent Pyroptosis[J].Drug Des Devel Ther,2022,16(1):413-423.
[10] 钟宝,万野,邹敏,等.颅脑损伤后血清IL-1β、IL6的变化及临床意义[J].中国实用神经疾病杂志,2018,21(2):184-187.
[11] 林树楷,刘仲海,刘珍,等.m6A RNA甲基化修饰在颅脑损伤模型大鼠坏死性凋亡中的作用[J].中国组织工程研究,2023,27(20):3167-3172.
[12] 刘玲玲,张红涛,王计伟,等.氢气对创伤性颅脑损伤大鼠大脑皮质NLRP3炎症小体的影响[J].中华危重病急救医学,2023,35(5):513-517.
[13] 张震,黄锦秀,李长生,等.瑞马唑仑预先给药对小鼠丘脑出血性脑损伤的影响[J].中华麻醉学杂志,2021,41(12):1518-1522.
[14] 徐倩倩,钱旭东,孙凡,等.西红花苷对缺血性脑卒中后抑郁大鼠炎症反应及TLR4/MyD88/NF-κB信号通路的影响[J].中国免疫学杂志,2021,37(2):179-185.
[15] Huang GR,Hao FG.Dexmedetomidine inhibits inflammation to alleviate early neuronal injury via TLR4/NF-κB pathway in rats with traumatic brain injury[J].Crit Rev Eukaryot Gene Expr,2021,1(1):41-47.