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| Establishment of a Guinea Pig Cholelithiasis Model Using Biliary Bacteria from Recurrent Common Bile Duct Stone Patients |
| BIAN Haoyu, HAN Ran, DUAN Xueguang, et al |
| Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China |
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Abstract Objective: To establish a guinea pig cholelithiasis model using biliary bacteria from patients with recurrent common bile duct stones, providing a clinically relevant animal model for investigating the role of biliary microbiota in the recurrence of common bile duct stones and the formation of cholelithiasis. Methods: Thirty-five Dunkin Hartley guinea pigs, regardless of gender, were used in two stages of experiments (20 in the first stage and 15 in the second stage). Biliary bacteria from patients with recurrent common bile duct stones were introduced by biliary catheterization. In the first stage, observations were made at the 9th week post-modeling, including postoperative survival rate, bile characteristics, and intrahepatic and extrahepatic stone formation in the animal model. Bile samples were collected for bacterial culture. In the second stage, two animals were sacrificed daily from the 2nd day post-modeling to observe bile characteristics and stone formation in the biliary system. Pathological changes in the liver, gallbladder, and common bile duct tissues of gallstone-bearing animals were examined, and the feasibility of the modeling method for establishing a cholelithiasis animal model was comprehensively evaluated. Results: Compared to previous modeling methods using biliary catheterization with injection of Escherichia coli combined with incomplete obstruction, the method used in this study was more consistent with the biliary microbial status of patients with recurrent common bile duct stones during stone recurrence and cholelithiasis formation. Moreover, it had a shorter modeling period, higher model success rate, relatively lower operational difficulty, and lower animal mortality rate. Conclusion: The modeling method used in this study successfully established a guinea pig cholelithiasis model, providing a reliable animal model for studying the mechanisms underlying the role of biliary microbiota in the recurrence of common bile duct stones and the formation of cholelithiasis after ERCP procedures.
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2024, Vol. 30 
