LncRNA PURPL调节miR-342-3p/PIK3R1轴对肝癌细胞恶性生物学行为的影响

doi:10.3969/j.issn.1006-6233.2024.01.05

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Abstract Objective: To investigate the impact of long non-coding RNA (PURPL) on the miR-342-3p/phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) axis and its effects on the malignant biological behavior of hepatocellular carcinoma (HCC) cells. Methods: Cell Experiments: Si-NC, si-PURPL, si-PURPL+inhibitor NC, si-PURPL+miR-342-3p inhibitor were separately transfected into HCC cells (Huh-7) and named si-NC group, si-PURPL group, si-PURPL+inhibitor NC group, si-PURPL+miR-342-3p inhibitor group, while untreated Huh-7 cells were designated as the NC group. Dual-luciferase reporter gene experiments verified the relationship between LncRNA PURPL, miR-342-3p, and PIK3R1. qRT-PCR was used to detect the expression of LncRNA PURPL and miR-342-3p in human normal liver cells (L02) and Huh-7 cells. Western blot assessed PIK3R1 protein levels in L02 and Huh-7 cells. MTT assay evaluated Huh-7 cell proliferation, flow cytometry measured apoptosis rates, Transwell assay determined invasive cell numbers, and scratch tests assessed cell migration. In Vivo Experiments: A xenograft mouse model was established with the same cell treatment groups. Tumor volume and weight were measured. Results: Cell experiment results showed that compared with the si-NC group, si-PURPL group had significantly decreased OD490 values, scratch healing rates, invasive cell numbers, LncRNA PURPL, and PIK3R1 levels in Huh-7 cells (P<0.05). Apoptosis rates and relative expression of miR-342-3p significantly increased (P<0.05). Inhibiting miR-342-3p expression weakened the inhibitory effects of silencing LncRNA PURPL on Huh-7 cell proliferation, migration, invasion, and promotion of apoptosis. LncRNA PURPL negatively regulated the miR-342-3p/PIK3R1 axis. In vivo results showed that the tumor volume and mass of si-PURPL group mice decreased compared to the si-NC group (P<0.05). Inhibiting miR-342-3p expression weakened the inhibitory effect of silencing LncRNA PURPL on tumor growth in vivo. Conclusion: Silting LncRNA PURPL can inhibit the malignant biological behavior of Huh-7 cells, and the mechanism may be related to the regulation of miR-342-3p/PIK3R1 axis.

Key words： LncRNA PURPL miR-342-3p/PIK3R1 axis Liver cancer Proliferation Apoptosis

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	WANG Hongmei
	CHEN Sirui
	DU Hong

Cite this article:

WANG Hongmei,CHEN Sirui,DU Hong. The Regulatory Effect of LncRNA PURPL on the miR-342-3p/PIK3R1 Axis and its Impact on the Malignant Biological Behavior of Hepatocellular Carcinoma Cells[J]. HeBei Med, 2024, 30(1): 29-35.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.01.05 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I1/29

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