三七白及粉通过Nrf2/Gpx4介导的铁死亡改善脑出血型应激性溃疡大鼠的胃黏膜损伤

doi:10.3969/j.issn.1006-6233.2023.08.07

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Abstract Objective: To investigate the protective effect of Panax notoginseng powder on gastric mucosal injury in rats with cerebral hemorrhagic stress ulcers via Nrf2/Gpx4-mediated iron death. Methods: A total of 60 rats were divided into NC group, SGU group, Panax notoginseng powder low dose group (Panax notoginseng powder L group), Panax notoginseng powder medium dose group (Panax notoginseng powder M group), Panax notoginseng powder high dose group (Panax notoginseng powder H group), Panax notoginseng powder high dose + Nrf2 specific inhibitor ML385 group (Panax notoginseng powder H + ML385 group) according to the random number table method, 10 rats in each group. The rats were scored for neurological function; the ulcer index of gastric mucosa was measured; HE staining was performed to detect histopathological changes in gastric tissues; Fe²⁺, MDA content and SOD activity were detected in gastric tissues; ROS levels in gastric tissues were detected by DHE fluorescence staining; Nrf2, Gpx4 and SLC7A11 protein expression in gastric mucosa tissues were detected by protein blotting. Results: Compared with the NC group, the neurological function score, gastric mucosal ulcer index, Fe²⁺, MDA content and ROS level increased and SOD activity decreased in the SGU group (P<0.05); compared with the SGU group, the neurological function score, gastric mucosal ulcer index, Fe²⁺, MDA content and ROS level decreased significantly and SOD activity increased in the Panax ginseng powder L, M and H groups ( P<0.05); compared with the Panax ginseng powder H group, the neurological function score, gastric mucosal ulcer index, Fe²⁺, MDA content and ROS level of rats in the Panax ginseng powder H+ML385 group were significantly higher and SOD activity was lower (P<0.05). Compared with the NC group, Nrf2, Gpx4 and SLC7A11 protein expression in the gastric mucosa tissues of rats in the SGU group were significantly decreased (P<0.05); compared with the SGU group, Nrf2, Gpx4 and SLC7A11 protein expression in the gastric mucosa tissues of rats in the Panax ginseng powder L, M and H groups were significantly increased in a dose-dependent manner (P<0.05); panax ginseng white and powder H+ Nrf2, Gpx4 and SLC7A11 protein expression in gastric mucosal tissues of rats in ML385 group were significantly lower than those in Panax notoginseng powder H groups (P<0.05). Conclusion: Panax notoginseng powder exerts a protective effect on gastric mucosal injury in rats with cerebral hemorrhagic stress ulcers, and the mechanism is related to the activation of Nrf2/Gpx4 pathway to inhibit iron death.

Key words： Panax notoginseng powder Nrf2/Gpx4 ron death Cerebral hemorrhagic stress ulcer Gastric mucosal injury

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.08.07 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I8/1267

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